摘要
Abstract
Objective:To investigate the improvement effects and mechanism of matrine(Mat)on inflammation and pulmo-nary fibrosis in rats with chronic obstructive pulmonary disease(COPD)based on the transforming growth factor-β1(TGF-β1)/signal transduction protein Smad3 pathway.Methods:The COPD model rats were established by the method of smoking combined with lipo-polysaccharide(LPS).The rats were grouped into COPD group,Mat group(100 mg/kg)and Mat+TGF-β1 group(100 mg/kg Mat+1.25 μg/kg TGF-β1),in addition,with 15 rats in each group.Another 15 randomly selected rats were set as a control group before modeling.Lung function analyzer was applied to detect the lung function of rats;HE staining was applied to observe the pathological changes of lung tissue in rats;the contents of TNF-α,IL-1β,TGF-β1 in the supernatant of alveolar lavage fluid(BALF)were detect-ed by ELISA;TUNEL staining was applied to observe apoptosis in lung tissue;immunohistochemistry was applied to detect the expres-sions of caspase-3 and matrix metalloproteinase-9(MMP-9)proteins in lung tissue;Western blot was applied to detect the expression of TGF/Smad3 pathway related proteins.Results:Compared with the control group,there were obvious inflammatory cell infiltration and cell exfoliation in the lung tissue of COPD rats,FVC,FEV0.1/FVC,FEV0.3/FVC obviously decreased(P<0.05);the levels of TNF-α,IL-1β,TGF-β1,the number of apoptotic cells,the expressions of caspase-3,MMP-9,TGF-β1,p-Smad3,Fibronectin,Col-lagen Ⅰ and α-SMA obviously increased(P<0.05);compared with COPD group,the pathological damage of lung tissue in Mat group was obviously improved,while FVC,FEV0.1/FVC and FEV0.3/FVC obviously increased(P<0.05);the levels of TNF-α,IL-1β,TGF-β1,the number of apoptotic cells,the expressions of caspase-3,MMP-9,TGF-β1,p-Smad3,Fibronectin,Collagen Ⅰ and α-SMA obviously increased(P<0.05);compared with Mat group,Mat+TGF-β1 group obviously reversed the improvement effect of Mat on COPD rats.Conclusion:Mat can improve pulmonary inflammation and fibrosis in COPD rats,which may be related to inhibiting the activation of TGF-β1/Smad3 pathway.关键词
苦参碱/慢性阻塞性肺疾病/转化生长因子-β1/信号传导蛋白Smad3通路/肺纤维化Key words
Matrine/Chronic obstructive pulmonary disease/Transforming growth factor-β1/signal transduction protein Smad3 pathway/Pulmonary fibrosis分类
医药卫生
