中国免疫学杂志2025，Vol.41Issue(6)：1420-1426,7.DOI:10.3969/j.issn.1000-484X.2025.06.027
卡介苗用于小鼠黑色素瘤模型免疫治疗的效应及其机制
Effect and mechanism of BCG immunotherapy in mice melanoma model
摘要
Abstract
Objective:To investigate immunotherapy effects and mechanism of BCG and recombinant BCG(rBCG)with c-di-AMP as adjuvant on melanoma in mice model.Methods:Melanoma mice model was established by B16F10 cell subcutaneous injec-tion in groin,and treated with 1×106 CFU of BCG and rBCG by adjacent injection of subcutaneous tumor for 3 times,respectively.Survival of melanotic mice,tumor growth and metastasis were observed.Tumor tissues of mice were isolated to prepare cell suspen-sion,and proportion of immune cells were detected by flow cytometry.Transcriptional levels of immune-related genes in tumor tissues were detected by qRT-PCR.Results:Both BCG and rBCG immunotherapy could significantly inhibit growth in melanoma mice and prolong survival time of mice.rBCG showed better inhibition on metastasis than BCG.Both strains significantly reduced proportion of M2-type macrophages and myeloid-derived suppressor cell associated with tumor growth and metastasis.Both two strains promoted infiltration of lymphocytes in tumor tissues,and rBCG significantly increased proportion of B cells in tumor.BCG immunotherapy upregulated transcription levels of metastasis-related cytokines,while rBCG therapy had no effects on transcriptions of these genes.Conclusion:Both BCG and rBCG have immunotherapeutic effects on melanotic mice,and rBCG with c-di-AMP as adjuvant shows better inhibition on tumor metastasis than BCG,which mechanism was related to regulation of immune response in tumor tissues.关键词
卡介苗/重组卡介苗/黑色素瘤/c-di-AMP/免疫治疗Key words
BCG/Recombinant BCG/Melanoma/c-di-AMP/Immunotherapy分类
医药卫生引用本文复制引用
徐铭泽,薛飒,柏银兰,宁唤唤,路延之,康健,彭钰君,张婧瑶,胡家豪,代婷,董梦娟..卡介苗用于小鼠黑色素瘤模型免疫治疗的效应及其机制[J].中国免疫学杂志,2025,41(6):1420-1426,7.基金项目
国家自然科学基金(81971560,82272343) （81971560,82272343）
陕西省重点研发计划(2022ZDLSF01-07). （2022ZDLSF01-07）
