中国免疫学杂志2025，Vol.41Issue(6)：1461-1466,6.DOI:10.3969/j.issn.1000-484X.2025.06.033
黄芩苷调节MIP-1α/CCR1信号通路对慢性偏头痛大鼠的治疗作用研究
Therapeutic effect of baicalin on chronic migraine rats by regulating MIP-1α/CCR1 signaling pathway
摘要
Abstract
Objective:To explore the therapeutic effect and mechanism of Baicalin(BG)on chronic migraine(CM)rats.Methods:CM rat model was established by intermittent subcutaneous injection of nitroglycerin.Rats were randomly grouped into con-trol group,chronic migraine group(CM group),low-dose and high-dose Baicalin groups(BG-L group,BG-H group),Baicalin+MIP-1α recombinant protein group(BG+MIP-1α group)and CCR1 inhibitor ZK811752 group(CCR1-ZK group),with 10 rats in each group.General behavior of rats were observed and scored;von Frey fiber optic pain gauge was applied to measure the mechanical pain threshold of rats;Nissl staining was applied to observe the neuronal structure of ventrolateral periaqueductal gray(vlPAG);ELISA was applied to detect levels of TNF-α and IL-1β;immunohistochemical method was applied to detect expressions of pain related pro-teins c-Fos and CGRP;Western blot was applied to detect expressions of MIP-1α and CCR1 proteins.Results:Compared with Control group,the body mass,mechanical pain threshold,and the number of Nissl bodies in vlPAG area of rats in CM group were obviously reduced,the general behavioral score,levels of TNF-α,IL-1β,and expressions of c-Fos,CGRP,MIP-1α and CCR1 were obviously increased(P<0.05);compared with CM group,the body mass,mechanical pain threshold,the number of Nissl bodies in vlPAG area of rats in BG-L group,BG-H group and CCR1-ZK group were obviously increased,the general behavioral score,levels of TNF-α,IL-1β,and expressions of c-Fos,CGRP,MIP-1α and CCR1 were obviously reduced(P<0.05);compared with BG-H group,the body mass,mechanical pain threshold,and the number of Nissl bodies in vlPAG area of rats in BG+MIP-1α group were obviously reduced,general behavioral score,levels of TNF-α,IL-1β,and expressions of c-Fos,CGRP,MIP-1α and CCR1 were obviously increased(P<0.05).Conclusion:Baicalin has certain therapeutic effect on chronic migraine rats,and its mechanism may be related to inhibiting the activation of MIP-1α/CCR1 signaling pathway.关键词
黄芩苷/巨噬细胞炎症蛋白-1α/趋化因子受体1信号通路/慢性偏头痛/机械痛阈值Key words
Baicalin/Macrophage inflammatory protein-1α/CC chemokine receptor 1 signaling pathway/Chronic migraine/Mechanical pain threshold分类
医药卫生引用本文复制引用
郭海峰,张晓燕,李菲,高琛,孙峰山..黄芩苷调节MIP-1α/CCR1信号通路对慢性偏头痛大鼠的治疗作用研究[J].中国免疫学杂志,2025,41(6):1461-1466,6.基金项目
山东省中医药科技发展计划项目(2019-0554). （2019-0554）
