中国临床药理学杂志2025，Vol.41Issue(8)：1072-1078,7.DOI:10.13699/j.cnki.1001-6821.2025.08.005
lncRNA MAGI1-IT1调节miR-485-5p/ACTG1轴对乳腺癌细胞生物学行为和上皮间质转化的影响
Effects of lncRNA MAGI1-IT1 on biological behavior and epithelial mesenchymal transformation of breast cancer cells by regulating miR-485-5p/ACTG1 axis
摘要
Abstract
Objective To investigate the effects of long non-coding RNA(lncRNA)MAGI1-IT1 on the proliferation,invasion,migration and epithelial mesenchymal transformation(EMT)of breast cancer cells by regulating the miR-485-5p/actin gamma 1(ACTG1)axis.Methods HCC1937 cells were cultured in vitro and randomly divided into control,si-MAGI1-IT1,miR-485-5p mimics,negative cotransfection and positive cotransfection groups.Control group was cultured normally without transfection;si-MAGI1-IT1 group was transfected with si-MAGI1-IT1;miR-485-5p mimics group was transfected with miR-485-5p mimics;negative cotransfection group was transfected with si-NC and NC-miR-485-5p;positive cotransfection group was given si-MAGI1-IT1 and miR-485-5p inhibitor combined transfection.Cell proliferation was detected by cell counting kit-8 method,cell migration was detected by cell scratch assay,cell invasion was detected by Transwell assay,and expression levels of lncRNA MAGI1-IT1,miR-485-5p and ACTG1 were detected by real-time fluorescence quantitative polymerase chain reaction method.The expression of proliferation and EMT-related proteins were detected by immunohistochemical staining.Results The cell activities(optical density)of the control,si-MAGI1-IT1,miR-485-5p mimics,negative cotransfection and positive cotransfection groups were 0.89±0.12,0.36±0.05,0.32±0.03,0.89±0.10 and 0.85±0.11;the migration rates were(91.82±11.13)％,(38.40±7.64)％,(33.17±6.03)％,(92.43±10.87)％and(86.10±10.76)％;the number of invasions was 296.50±23.48,93.00±17.36,76.50±15.01,302.50±25.32 and 269.00±24.92;the relative expression levels of lncRNA MAGI1-IT1 were 0.98±0.11,0.29±0.06,0.96±0.10,1.00±0.12 and 0.30±0.07;the relative expression levels of miR-485-5p were 0.99±0.13,2.12±0.16,2.20±0.18,0.98±0.15 and 1.04±0.16;the relative expression levels of ACTG1 were 1.01±0.12,0.34±0.05,0.31±0.07,1.02±0.14 and 0.97±0.11;the positive expression levels of proliferating cell nuclear antigen(optical density)were 1.65±0.16,0.53±0.12,0.45±0.08,1.64±0.14 and 1.60±0.17;the positive expression levels of Cyclin D1(optical density)were 1.53±0.15,0.46±0.11,0.40±0.06,1.52±0.14 and 1.48±0.13;the positive expression levels of Vimentin(optical density)were 1.49±0.14,0.45±0.07,0.39±0.06,1.50±0.12 and 1.45±0.13,respectively.The above indexes in the si-MAGI1-IT1 group were compared with the control group,the above indexes in the miR-485-5p mimics group(except lncRNA MAGI 1-IT1)were compared with the control group,the above indexes in the positive cotransfection group(except lncRNA MAGI1-IT1)were compared with the si-MAGI1-IT1 group,the differences were statistically significant(all P＜0.05).Conclusion Knockdown of lncRNA MAGI1-IT1 can reduce the expression of ACTG1 by up-regulating miR-485-5p,thereby inhibiting the proliferation,migration,invasion and EMT of breast cancer cells.关键词
长链非编码RNA MAGI1-IT1/miR-485-5p/肌动蛋白γ1/乳腺癌/增殖/侵袭/迁移Key words
long non-coding RNA MAGI1-IT1/miR-485-5p/actin gamma 1/breast cancer/proliferation/invasion/migration分类
医药卫生引用本文复制引用
王峰,王文彦,曲更宝,朱强..lncRNA MAGI1-IT1调节miR-485-5p/ACTG1轴对乳腺癌细胞生物学行为和上皮间质转化的影响[J].中国临床药理学杂志,2025,41(8):1072-1078,7.基金项目
北京市优秀人才培养基金资助项目(2016000021469G211) （2016000021469G211）
北京市青年骨干项目基金资助项目(2016000021469G211) （2016000021469G211）
