中国临床药理学杂志2025，Vol.41Issue(8)：1079-1084,6.DOI:10.13699/j.cnki.1001-6821.2025.08.006
吡咯替尼通过LncRNA SNHG16调控p53/SLC7A11轴诱导胃癌细胞铁死亡的研究
Research of pyrotinib inducing ferroptosis in gastric cancer cells through LncRNA SNHG16 regulation of the p53/SLC7A11 axis
摘要
Abstract
Objective To investigate the effect of pyrotinib on ferroptosis in gastric cancer cells through the regulation of the tumor protein 53(p53)/solute carrier family 7 member 11(SLC7A11)axis by the long noncoding RNA small nucleolar RNA host gene 16(LncRNA SNHG16).Methods Human gastric cancer cells(NCI-N87)were selected and divided into NCI-N87 group(NCI-N87 cells were cultured normally without treatment),experimental group(NCI-N87 cells treated with 0.5 μmol·L-1 pyrotinib for 72 h),and oe-NC group(based on the transfection of oe-NC in experimental group),LncRNA SNHG16 group(based on experimental group transfected with oe-LncRNASNHG16).Real-time fluorescence quantitative polymerase chain reaction and fluorescence in situ hybridisation were used to determine the expression level of LncRNA SNHG16,cell viability was detected by cell counting kit-8;invasive ability was detected by Transwell assay;reactive oxygen species(ROS)fluorescence level was detected by fluorescent probe staining of dihydroethidium(DHE);malondialdehyde(MDA)level and glutathione(GSH)activity were detected by kits;and the expression level of p53 and SLC7A11 protein was detected by Western blot.Results The relative expression levels of LncRNA SNHG16 in the NCI-N87 group,the experimental group,the oe-NC group and the LncRNA SNHG16 group were 1.00±0.18,0.39±0.06,0.41±0.07 and 0.80±0.15,respectively;the relative fluorescence intensities of LncRNA SNHG16 were 1.00±0.16,0.27±0.05,0.23±0.04 and 0.81±0.17,respectively;the number of cell invasion was(82.16±14.67),(29.03±4.75),(31.82±5.69)and(74.98±11.34)pieces,respectively;the relative fluorescence levels of ROS were 1.00±0.15,4.57±0.86,3.95±0.61 and 1.54±0.33,respectively;MDA levels were(5.49±1.08),(11.27±1.93),(10.85±1.64)and(6.73±0.95)μmol·mg-1,respectively;GSH relative activities were 1.00±0.00,0.54±0.09,0.56±0.07 and 0.83±0.14,respectively;the relative expression levels of p53 protein were 1.00±0.17,2.26±0.38,1.98±0.32 and 1.24±0.19,respectively;the relative expression levels of SLC7A11 protein were 1.00±0.13,0.34±0.05,0.41±0.09 and 0.79±0.11,respectively.The differences in the above indicators between the NCI-N87 group and the experimental group,as well as between the oe-NC group and the LncRNA SNHG16 group,were all statistically significant(P＜0.001).Conclusion Pyrotinib can regulate the p53/SLC7A11 signaling pathway by inhibiting the expression of LncRNA SNHG16 to induce ferroptosis of gastric cancer cells and inhibit the proliferation and invasion of gastric cancer cells.关键词
吡咯替尼/长链非编码RNA小核仁RNA宿主基因16/肿瘤蛋白p53/溶质载体家族7成员11/胃癌/铁死亡/增殖/侵袭Key words
pyrotinib/long noncoding RNA small nucleolar RNA host gene 16/tumor protein 53/solute carrier family 7 member 11/gastric cancer/ferroptosis/proliferation/invasion分类
医药卫生引用本文复制引用
刘承一,胡潺潺,李青山,肇爽,陈慧,李智慧,焦海静,殷亚兰..吡咯替尼通过LncRNA SNHG16调控p53/SLC7A11轴诱导胃癌细胞铁死亡的研究[J].中国临床药理学杂志,2025,41(8):1079-1084,6.基金项目
河北省医学科学研究重点课题计划基金资助项目(20210440) （20210440）
