中国临床药理学杂志2025，Vol.41Issue(8)：1085-1090,6.DOI:10.13699/j.cnki.1001-6821.2025.08.007
依托咪酯调控mTOR/SIRT1表达抑制膀胱癌BIU-87细胞恶性生物学行为的研究
Research of etomidate inhibiting the malignant biological behavior of bladder cancer BIU-87 cells by regulating the expression of mTOR/SIRT1
摘要
Abstract
Objective To investigate the effects of etomidate on the expression of mammalian sirolimus like target protein(mTOR)/silencing information regulatory factor 1(SIRT1)gene to inhibit the proliferation,invasion and migration of bladder cancer BIU-87 cells.Methods The BIU-87 cells were divided into normal group,positive control group and experimental-L,-M,-H groups.The normal group was given normal culture;the positive control group was treated with MEM medium containing paclitaxel at a final concentration of 15 mg·mL-1;the experimental-L,-M,-H groups were treated with MEM medium containing etomidate at final concentrations of 112.50,225.00 and 450.00 mg·L-1,respectively.After 24 hours of intervention,the migration ability of cells was detected using the cell scratching method,the invasion ability of cells was detected using the Transwell method,the levels of glucose and lactate in cells were detected using enzyme-linked immunosorbent assay,and the expression levels of mTOR,SIRT1 and matrix metalloproteinase(MMP)proteins were detected using Western blotting.Results The number of invasive cells in the experimental-M,-H groups,positive control group and normal group was 248.56±5.67,117.37±16.72,76.54±8.23 and 376.40±53.41;the migration distances were(22.30±2.89),(13.67±5.46),(8.63±2.07)and(38.24±5.24)μm;the glucose levels were(38.97±2.43),(29.75±3.58),(21.52±4.20)and(64.26±7.26)μmol·L-1;the lactate levels were(37.36±3.29),(20.32±5.67),(14.76±2.37)and(51.52±3.10)μmol·L-1;the phosphorylated mTOR/mTOR ratios were 0.26±0.05,0.20±0.04,0.15±0.03 and 0.47±0.07;the relative expression levels of SIRT1 protein were 0.23±0.01,0.18±0.02,0.09±0.02 and 0.72±0.13;the relative expression levels of MMP-9 protein were 0.19±0.05,0.14±0.01,0.10±0.01 and 0.28±0.04,respectively.Compared with positive control group and normal group,the differences of above indexes in experimental-M,-H groups were statistically significant(all P＜0.05).Conclusion Etomidate can effectively inhibit the proliferation,invasion and migration of BIU-87 cells by suppressing the expression of mTOR/SIRT1.关键词
依托咪酯/哺乳动物类西罗莫司靶蛋白/沉默信息调节因子1/膀胱癌/细胞增殖/细胞侵袭/细胞迁移Key words
etomidate/mammalian target of rapamycin/silencing information regulator 1/bladder cancer/cell proliferation/cell invasion/cell migration分类
医药卫生引用本文复制引用
李晓石,李世宾,张慧明,杨文博..依托咪酯调控mTOR/SIRT1表达抑制膀胱癌BIU-87细胞恶性生物学行为的研究[J].中国临床药理学杂志,2025,41(8):1085-1090,6.基金项目
河南省医学科技攻关计划基金资助项目(LHGJ20230885) （LHGJ20230885）
