中国临床药理学杂志2025，Vol.41Issue(8)：1091-1095,5.DOI:10.13699/j.cnki.1001-6821.2025.08.008
羟基红花黄色素A对慢性阻塞性肺疾病急性加重期小鼠中性粒细胞迁移的影响
Effects of hydroxysafflor yellow A on neutrophil migration in acute exacerbation of chronic obstructive pulmonary disease mice
摘要
Abstract
Objective To explore the therapeutic effect of hydroxysafflor yellow A(HSYA)on acute exacerbation of chronic obstructive pulmonary disease(AECOPD)mice and its mechanism of action.Methods Mice were randomly divided into control group,model group and experimental-L,-M,-H groups,with 20 mice per group.The control group was raised normally,and the other groups were exposed to cigarette smoke(CS)and non-typeable Haemophilus influenzae(NTHi)infection to establish an AECOPD mouse model.Starting from the 12th week of CS exposure,experimental-L,-M,-H groups were administered 25,50 and 100 mg·kg-1·d-1 HSYA by gavage,and control and model groups were given 0.9％NaCl by gavage.Five groups were administered once a day for 2 weeks.The functional residual capacity(FRC)of the mice was measured using the Buxco lung function test system.The percentage of neutrophils in bronchoalveolar lavage fluid(BALF)was measured using flow cytometry.The expression levels of phosphatidylinositol-3-kinase(PI3K)and protein kinase B(Akt)in lung tissues were detected by Western blot.Results The FRC of experimental-M,-H groups,model group and control group were(0.31±0.01),(0.27±0.01),(0.37±0.02)and(0.25±0.01)L;the neutrophil percentages were(37.96±2.88)％,(28.41±1.64)％,(60.27±2.12)％and(11.33±0.31)％;the phosphorylated PI3K/PI3K ratios were 1.59±0.08,1.30±0.06,2.28±0.08 and 1.00±0.06;the phosphorylated Akt/Akt ratios were 1.33±0.11,1.15±0.03,1.76±0.11 and 1.00±0.06,respectively.Compared with model group,the differences of above indexes in experimental-M,-H groups were statistically significant(all P＜0.05).Conclusion HSYA may reduce neutrophil activity by inhibiting the PI3K/Akt pathway,thereby inhibiting inflammatory responses and alleviating lung injury.关键词
羟基红花黄色素A/慢性阻塞性肺疾病急性加重期/中性粒细胞迁移/炎症反应/磷脂酰肌醇-3-激酶/蛋白激酶B途径Key words
hydroxysafflor yellow A/acute exacerbation of chronic obstructive pulmonary disease/neutrophil migration/inflammation response/phosphatidylinositol-3-kinase/protein kinase B pathway分类
医药卫生引用本文复制引用
王忠芳,吉自敏,林志强,李永兵,吕建华..羟基红花黄色素A对慢性阻塞性肺疾病急性加重期小鼠中性粒细胞迁移的影响[J].中国临床药理学杂志,2025,41(8):1091-1095,5.基金项目
河南省医学科技攻关计划联合共建基金资助项目(LHGJ20221015) （LHGJ20221015）
