中国渔业质量与标准2025，Vol.15Issue(1)：10-18,9.DOI:10.3969/j.issn.2095-1833.2025.01.002
现场快速筛查即食海参中副溶血弧菌污染的方法
A Simple and Rapid Method for the Screening of Vibrio parahaemolyticus Contamination in Ready-to-Eat Sea Cucumber On-site
摘要
Abstract
In this study,based on an electronic microbial growth analyzer,a simple and rapid method for the quantita-tive screening of viable Vibrio parahaemolyticus.contamination in ready-to-eat sea cucumber products was devel-oped.The liquid companion was sampled from the package of ready-to-eat sea cucumber product,and was directly transferred into a test tube,in which TCBS broth was preloaded.The test tube was then positioned in the electronic microbial growth analyzer to determine the bacterial growth curve,growth rate curve and the time required to reach the maximum growth rate(Tmgr).Using the QC strain as a model Vibrio parahaemolyticus,there was linear relationship between the logarithmic value of viable bacterial concentration(log C)and Tmgr over the range of 5×101～5×105 CFU/mL,with a detection limit of 101 CFU/mL.The regression equation was Tmgr(min)=-48.822 log C(CFU/mL)+443.05(r=0.999 2).When Vibrio parahaemolyticus contamination reached 5.0×104 CFU/mL,the recovery rates were 89.37%to 96.06%.The total time consumption,from the unwrapping of the package of ready-to-eat sea cucumber product to the readout of bacterial concentration,was no more than 4 h.This new method re-quires only a portable instrument and three simple manual steps,making it suitable for on-site analysis.The pro-posed method represents a promising alternative for the on-site screening and quantification of viable Vibrio spp.in ready-to-eat sea cucumber products.[Chinese Fishery Quality and Standards,2025,15(1):10-18].关键词
即食海参/弧菌污染/定量筛查/现场快检/电子微生物生长分析仪Key words
ready-to-eat sea cucumber/Vibrio contamination/quantitative screening/on-site and rapid detec-tion/electronic microbial growth analyzer分类
轻工业引用本文复制引用
王笑旸,卢峰,朱雪婷,孔青,杨倩倩,张旭志..现场快速筛查即食海参中副溶血弧菌污染的方法[J].中国渔业质量与标准,2025,15(1):10-18,9.基金项目
国家重点研发计划项目(2024YFD2400402) （2024YFD2400402）
