中国药物经济学2025，Vol.20Issue(5)：103-107,5.DOI:10.12010/j.issn.1673-5846.2025.05.022
板蓝根中4种成分的含量测定及质量评价
Content Determination and Quality Evaluation of Four Constituents in Isatis Indigotica
摘要
Abstract
Objective To establish a method for the simultaneous determination of the contents of four components in Isatidis Indigotica samples by high performance liquid chromatography(HPLC),and to evaluate the quality of Isatidis Indigotica.Methods The content determination of four components,namely(R,S)-hyacinol,guanosine,uridine and adenosine,was established by HPLC.Cluster analysis and Pearson correlation analysis were conducted on the contents of the four components in the Isatis Indigotica samples.Results The established HPLC for one test and multiple evaluations can be used for the simultaneous determination of four components(R,S)in Isatidis Radix-acanthopterine,guanosine,uridine and adenosine.Pearson correlation analysis revealed that there were partial correlations among the four components in Isatidis Indigotica.Among them,(R,S)-Aconitum was negatively correlated with the content of uridine,and the content of uridine was positively correlated with that of guanosine.Conclusion The establishment of an HPLC for the simultaneous determination of four components features high sensitivity,strong specificity,good precision,and excellent repeatability and reliability.Combined with stoichiometric analysis,it can reflect the differences in the quality of Isatidis Indigotica as a whole,comprehensively and truly,and has certain guiding significance for the quality control and overall evaluation of Isatidis Indigotica.关键词
板蓝根/(R,S)-告依春/鸟苷/尿苷/腺苷/聚类分析Key words
Isatidis Radix/(R,S)-goitrin/Uridine/Guanosine/Adenosine/Cluster analysis分类
医药卫生引用本文复制引用
刘柏龙,夏巧红,刘生彩,张明童,刘小燕,马潇..板蓝根中4种成分的含量测定及质量评价[J].中国药物经济学,2025,20(5):103-107,5.基金项目
甘肃省药品监督管理局课题(2022GSMPA0018) （2022GSMPA0018）
国家药品监督管理局中药材及饮片质量控制重点实验室项目(2021GSMPA-ΚL07) （2021GSMPA-ΚL07）
