针灸推拿医学（英文版）2025，Vol.23Issue(3)：197-209,13.DOI:10.1007/s11726-025-1502-4
艾灸天枢和气海对克罗恩病肠纤维化小鼠结肠ACE/Ang Ⅱ/AT1R轴的影响
Effects of moxibustion at Tianshu(ST25)and Qihai(CV6)on ACE/Ang Ⅱ/AT1R axis in the colon tissue of Crohn disease mice with intestinal fibrosis
摘要
Abstract
Objective:To explore the therapeutic mechanism of moxibustion in Crohn disease(CD)-associated intestinal fibrosis by observing its effects on the angiotensin-converting enzyme(ACE)/angiotensin Ⅱ(Ang Ⅱ)/angiotensin Ⅱ type 1 receptor(AT1R)axis in CD mouse models. Methods:Six randomly selected male C57BL/6 mice were assigned to a normal group,while the remaining mice were administered 0.1 mL of 2,4,6-trinitrobenzene sulfonic acid via enema to establish a CD intestinal fibrosis model.After successful modeling,the mice were randomly divided into a model group,a moxibustion group,and a Western medication group,with 6 rats in each group.The normal group and the model group only received grabbing without intervention.In the moxibustion group,mild moxibustion was applied to Qihai(CV6)and bilateral Tianshu(ST25)once a day for 10 min each time over 7 consecutive days.The Western medication group was administered mesalazine suspension via oral gavage once a day for 7 consecutive days.At the end of the intervention,the general condition,disease activity index(DAI)score,and gross colon score of mice in each group were evaluated.Hematoxylin-eosin staining was used to observe and score the histological changes in the colon tissue in each group.Masson staining was used to observe colonic fibrosis and the ratio of collagen-positive areas was analyzed;the expression of Ang Ⅱ in the colon tissue was detected by the enzyme-linked immunosorbent assay;immunohistochemistry and real-time quantitative reverse transcription polymerase chain reaction were used to detect the protein and mRNA expression of ACE and AT1R in the colon tissue,respectively;Western blotting was used to detect the expression of transforming growth factor(TGF)-β1 and connective tissue growth factor(CTGF)in the colon tissue. Results:Compared to the normal group,the DAI score,gross colon score,colonic histological score,collagen-positive area ratio,ACE protein and mRNA,Ang Ⅱ protein,AT1R protein and mRNA,TGF-β1 protein,and CTGF protein in the colon tissue in the model group increased significantly(P<0.01).In contrast,the above indicators in both the moxibustion group and the Western medication group reduced significantly compared to the model group(P<0.01 or P<0.05).There was no statistical difference in these indicators between the moxibustion group and the Western medication group(P>0.05). Conclusion:Moxibustion can alleviate intestinal fibrosis in CD mice,and its therapeutic mechanism may be associated with the regulation of colonic ACE/AngⅡ/AT1R axis.关键词
艾条灸/穴,天枢/穴,气海/克罗恩病/血管紧张素转换酶/血管紧张素Ⅱ/血管紧张素Ⅱ 1型受体/小鼠Key words
Moxa Stick Moxibustion/Point,Tianshu(ST25)/Point,Qihai(CV6)/Crohn Disease/Angiotensin-converting Enzyme/Angiotensin Ⅱ/Angiotensin Ⅱ Type 1 Receptor/Mice分类
医药卫生引用本文复制引用
彭洋,高倩倩,冯芊茹,朱笑吉,马喆,吴焕淦,刘慧荣,周次利..艾灸天枢和气海对克罗恩病肠纤维化小鼠结肠ACE/Ang Ⅱ/AT1R轴的影响[J].针灸推拿医学（英文版）,2025,23(3):197-209,13.基金项目
This work was supported by the Projects of National Natural Science Foundation of China(国家自然科学基金项目,No.81873377,No.82374588) （国家自然科学基金项目,No.81873377,No.82374588）
Project of Shanghai Municipal Natural Science Foundation(上海市自然科学基金项目,No.21ZR1460000) （上海市自然科学基金项目,No.21ZR1460000）
Health Industry Clinical Research Special Projects of Shanghai Municipal Health Commission(上海市卫生健康委员会卫生行业临床研究专项项目,No.202040250,No.202140113) （上海市卫生健康委员会卫生行业临床研究专项项目,No.202040250,No.202140113）
"Xinglin Hundred Talents"Program of Shanghai University of Traditional Chinese Medicine(上海中医药大学"杏林百人"计划) （上海中医药大学"杏林百人"计划）
Graduate Innovation Training Program of Shanghai University of Traditional Chinese Medicine(上海中医药大学研究生创新培养专项,No.Y2020040). （上海中医药大学研究生创新培养专项,No.Y2020040）
