针灸推拿医学（英文版）2025，Vol.23Issue(3)：218-230,13.DOI:10.1007/s11726-025-1504-2
艾灸任脉、督脉和冲脉穴位对寒凝血瘀大鼠下丘脑-垂体-卵巢轴的影响
Effects of moxibustion at points from the Conception,Governor,and Thoroughfare Vessels on hypothalamic-pituitary-ovary axis in rats with cold-induced blood stasis pattern
摘要
Abstract
Objective:To explore the mechanism by which moxibustion improves cold-induced blood stasis pattern(CIBSP)and to uncover the biological basis underlying the correlation between the Conception Vessel(CV),Governor Vessel(GV),Thoroughfare Vessel(TV),and the uterus by investigating the effects of mild moxibustion on the hypothalamic-pituitary-ovary axis(HPOA)in CIBSP model rats. Methods:Forty-eight female Sprague-Dawley rats were divided into a blank group,a model group,a CV group,a GV group,a TV group,and a non-meridian non-point(NMNP)group according to the random number table method,with 8 rats in each group.Except for rats in the blank group,the other rats were subjected to an ice water bath plus epinephrine to prepare CIBSP models.Each intervention group was given mild moxibustion on the first day of modeling.Qihai(CV6),Guanyuan(CV4),and Zhongji(CV3)were selected in the CV group;Huangshu(KI16),Dahe(KI12),and Henggu(KI11)were selected in the TV group;Xuanshu(GV5),Mingmen(GV4),and Yaoyangguan(GV3)were selected in the GV group;three NMNP spots were selected in the NMNP group.Mild moxibustion was administered for 20 min each time,once a day,for 14 consecutive days.The symptom scores of CIBSP and uterine electromyogram were compared among groups;morphological changes in reproductive organs were observed by hematoxylin-eosin staining;enzyme-linked immunosorbent assay was used to detect serum reproductive hormones and neurotransmitters.The mRNA and protein expression levels of estrogen receptor(ER),follicle-stimulating hormone receptor(FSHR),and luteinizing hormone receptor(LHR)were detected by real-time quantitative polymerase chain reaction,immunohistochemistry,or Western blotting assays. Results:Compared to the blank group,the model group showed a significantly increased CIBSP symptom score,an amplified in vivo uterine myoelectric value;decreased ovarian follicles,thinner endometrial epithelium,and disordered structure with the pathological examination,decreased serum levels of reproductive hormones,6-keto-prostaglandin F1α(6-keto-PGF1α),and β-endorphin(β-EP),increased thromboxane B2(TXB2),5-hydroxytryptamine(5-HT),and substance P(SP),and decreased mRNA and protein expression of ovarian ER,FSHR,and LHR(P<0.05 or P<0.01).Compared to the model and NMNP groups,each point-intervention group showed a decreased CIBSP symptom score and uterine myoelectric value;increased ovarian follicles,thickened endometrium,increased glands,up-regulated serum reproductive hormones,6-keto-PGF1α,and β-EP,down-regulated serum TXB2,5-HT,and SP and increased mRNA and protein expression of ovarian ER,FSHR,and LHR(P<0.05 or P<0.01). Conclusion:Moxibustion at points from the CV,GV,and TV("three branches from one source")can improve the reproductive neuroendocrine function of CIBSP rats,and this effect may be mediated through the regulation of the HPOA.These detections suggest that the biological basis of point-uterus correlation may be based on HPOA regulation.关键词
灸法/一源三歧/寒凝血瘀证/下丘脑-垂体-卵巢轴/生殖激素受体/经穴-胞宫相关/大鼠Key words
Moxibustion Therapy/Three Branches from One Source/Cold-induced Blood Stasis Pattern/Hypothalamic-pituitary-ovary Axis/Reproductive Hormone Receptor/Acupuncture Point-uterine Correlation/Rats分类
医药卫生引用本文复制引用
张璇,刘佳欣,李新华,王迪,佘延芬,任艳青,成秀梅..艾灸任脉、督脉和冲脉穴位对寒凝血瘀大鼠下丘脑-垂体-卵巢轴的影响[J].针灸推拿医学（英文版）,2025,23(3):218-230,13.基金项目
This work was supported by the Youth Project of National Natural Science Foundation of China(国家自然科学基金青年科学基金项目,No.82104740) （国家自然科学基金青年科学基金项目,No.82104740）
Youth Project of Natural Science Foundation of Hebei Province(河北省自然科学基金青年科学基 金项目,No.H2022423028) （河北省自然科学基金青年科学基 金项目,No.H2022423028）
General Project of National Natural Science Foundation of China(国家自然科学基金面上项目,No.8197150107) （国家自然科学基金面上项目,No.8197150107）
Scientific Research Program of Hebei Provincial Administration of Traditional Chinese Medicine(河北省中医药管理局科研计划项目,No.2021097) （河北省中医药管理局科研计划项目,No.2021097）
Project of Natural Science Foundation of Hebei Province(河北省自然科学基金项目,No.H2022423368). （河北省自然科学基金项目,No.H2022423368）
