针灸推拿医学（英文版）2025，Vol.23Issue(3)：231-238,8.DOI:10.1007/s11726-025-1505-1
艾灸对佐剂型关节炎大鼠膝关节滑膜组织S100A8,S100A9与SAA1蛋白表达的影响
Effects of moxibustion on protein expression of S100A8,S100A9,and SAA1 in knee joint synovial tissue of rats with adjuvant arthritis
摘要
Abstract
Objective:To investigate the protective effect of moxibustion on joints and its influence on the expression levels of S100 calcium binding protein A8(S100A8),S100 calcium binding protein A9(S100A9),serum amyloid A1(SAA1),and related inflammatory factors in rats with adjuvant arthritis(AA). Methods:Forty Wistar rats were randomly divided into a normal group,a model group,a moxibustion group,and a medication group,with 10 rats in each group.Except for the normal group,AA models were established in the other three groups by exposing rats to wind-cold-dampness environmental conditions combined with complete Freund's adjuvant.After successful modeling,the moxibustion group received moxibustion intervention,while the medication group was administered tripterygium glycosides tablets via oral gavage.The normal and model groups underwent similar handling and fixation without additional interventions.After 15 d of intervention,hematoxylin-eosin staining was used to assess pathological changes in the knee joint synovial membrane.Western blotting was performed to detect the protein expression of S100A8,S100A9,and SAA1 in the synovial tissue.Enzyme-linked immunosorbent assay was used to measure the serum levels of interferon(IFN)-γ,interleukin(IL)-6,and IL-23. Results:Compared to the normal group,the model group exhibited significantly increased protein expression of S100A8,S100A9,and SAA1 in the knee joint synovial tissue,as well as elevated serum levels of IFN-γ,IL-6,and IL-23(P<0.01).Histopathological analysis revealed marked synovial hyperplasia and extensive infiltration of inflammatory cells in the model group.Compared to the model group,both the moxibustion and medication groups showed significant reductions in the protein expression of S100A8,S100A9,and SAA1 in the synovial tissue,as well as decreased serum levels of IFN-γ,IL-6,and IL-23(P<0.01).Additionally,synovial tissue in these two groups displayed minimal hyperplasia and only mild inflammatory cell infiltration.Notably,compared to the moxibustion group,the medication group exhibited significantly higher protein expression of S100A9 in the synovial tissue(P<0.05),while no significant differences were observed in the expression of S100A8,SAA1,or serum levels of IFN-γ,IL-6,and IL-23(P>0.05).Both intervention groups showed comparable degrees of synovial inflammation,clear tissue structure,and no obvious hyperplasia. Conclusion:Moxibustion can alleviate joint swelling and reduce inflammatory responses in AA rats.Its mechanism may involve regulating the protein expression of S100A8,S100A9,and SAA1 in the knee joint synovial tissue.关键词
针灸疗法/艾条灸/关节炎,实验性/钙结合蛋白/血清淀粉样蛋白A/干扰素-γ/白细胞介素类/大鼠Key words
Acupuncture-moxibustion Therapy/Moxa Stick Moxibustion/Arthritis,Experimental/Calcium-binding Proteins/Serum Amyloid A Protein/Interferon-γ/Interleukins/Rats分类
医药卫生引用本文复制引用
臧乃丰,汪英杰,孙奎,彭传玉,王天城,刘新悦,邵雅文,吴子建,魏霞,朱抗洪,杨柳..艾灸对佐剂型关节炎大鼠膝关节滑膜组织S100A8,S100A9与SAA1蛋白表达的影响[J].针灸推拿医学（英文版）,2025,23(3):231-238,8.基金项目
This work was supported by Youth Project of National Natural Science Foundation of China(国家自然科学基金青年科学基金项目,No.82205289) （国家自然科学基金青年科学基金项目,No.82205289）
Key Project of Natural Science Research Foundation for Universities in Anhui Province(安徽省高校自然科学研究基金重点项目,No.KJ2021A0571) （安徽省高校自然科学研究基金重点项目,No.KJ2021A0571）
Youth Project of Natural Science Foundation of Anhui Province(安徽省自然科学基金青年项目,No.2208085QH265). （安徽省自然科学基金青年项目,No.2208085QH265）
