中医药学报2025，Vol.53Issue(6)：26-31,6.DOI:10.19664/j.cnki.1002-2392.250113
覆盆子原花青素对雄性糖尿病桑蚕生殖损伤的修复作用
Reparative Effect of Raspberry Proanthocyanidins on Reproductive Damage in Male Diabetic Silkworms
摘要
Abstract
Objective:To investigate the reparative effect of raspberry proanthocyanidins on reproductive damage in male diabetic silkworms.Methods:Silkworms in the experimental group were fed a high-glucose diet containing 10%glucose for 18 hours to establish a type 2 diabetes model.The diabetic silkworms were randomly divided into a model group,and high-dose(50 μL),medium-dose(25 μL),and low-dose raspberry proanthocyanidins(12.5 μL)groups.The levels of testosterone(T),luteinizing hormone(LH),and follicle-stimulating hormone(FSH)in the hemolymph were measured;triglyceride(TG)levels in the fat body were determined;superoxide dismutase(SOD),malondialdehyde(MDA),and glutathione peroxidase(GSH-Px)levels in the testicular tissue were measured;pathological damage to testicular tissue was observed via HE staining;and the expression levels of siwi1 and siwi2 genes in the PiRNA signaling pathway were analyzed using RT-PCR.Results:Compared to the model group,all raspberry proanthocyanidin doses led to a reduction in blood glucose levels(P<0.05)and an increase in LH levels(P<0.05),with high-and medium-dose groups also showing increased T levels(P<0.05).The levels of GSH-Px and SOD were elevated(P<0.05),while MDA levels were decreased(P<0.05)in all raspberry proanthocyanidin groups.Testicular pathological damage was alleviated,and mRNA levels of siwi1 and siwi2 were increased(P<0.05)in all raspberry proanthocyanidin groups.Conclusion:Raspberry proanthocyanidins can improve male reproductive damage caused by diabetes by lowering blood glucose and lipid levels,increasing LH and T levels,reducing oxidative stress in the testes,alleviating testicular pathological damage,and enhancing the expression of related genes in the PiRNA signaling pathway.关键词
覆盆子原花青素/糖尿病桑蚕/生殖损伤/修复作用Key words
Raspberry proanthocyanidins/Diabetic silkworms/Reproductive damage/Repair action分类
中医学引用本文复制引用
曾小艳,童丽..覆盆子原花青素对雄性糖尿病桑蚕生殖损伤的修复作用[J].中医药学报,2025,53(6):26-31,6.基金项目
"昆仑英才·高端创新创业人才"领军人才项目(青人才字[2021]9号) （青人才字[2021]9号）
