中国肿瘤生物治疗杂志2025，Vol.32Issue(5)：492-497,6.DOI:10.3872/j.issn.1007-385x.2025.05.006
国产与进口CD3/CD28激活磁珠制备CAR-T细胞的性能对比
Performance comparison of domestic and imported CD3/CD28 activation beads for CAR-T cell manufacturing
摘要
Abstract
Objective:To compare the performance differences of domestic and imported CD3/CD28 activation beads for manufacturing CAR-T cells,providing a backup or alternative for domestic CAR-T cell research and manufacture.Methods:A mature protocol using imported CD3/CD28 activation beads with a 1∶1 ratio for CD3+T cells was implemented as research control.Domestic beads were used with gradient ratios of 1∶2,1∶1,and 2∶1 to activate T cells.72 h after T cell activation,CAR-T cells were manufactured by CAR lentiviral infection and cell proliferation was monitored at 2-,4-,and 7-days post-infection.Flow Cytometry was used to detect CAR-T cell positivity 5 days after infection and to detectCD4/CD8 phenotype of CAR-T cells and PD1+TIM3+cell exhaustion ratio 8 days after infection.Results:CAR-T cells manufactured by domestic CD3/CD28 activation beads exhibited similar phenotype compared with those manufactured by imported CD4/CD8 beads.The positive rate of CAR-T cells prepared with domestic beads was slightly lower than that of imported beads(53.7%vs 57.9%).However,the proliferation of CAR-T cells manufactured by domestic beads was about twice that of cells manufactured by imported beads,and the exhaustion level was only half that of imported beads(4.21%vs 7.91%).Moreover,the use of domestic magnetic beads was lower than that of imported magnetic beads,which was advantageous for cutting the costs of CAR-T cells research and manufacture.Conclusion:Domestic CD3/CD28 activation beads used for CAR-T cells manufacturing demonstrate comparable overall performance to their imported counterparts,showing potential as a backup or alternative for imported beads.关键词
CD3/CD28激活磁珠/CAR-T细胞制备/性能对比/国产替代Key words
CD3/CD28 activation beads/CAR-T cell manufacturing/performance comparison/domestic alternatives分类
临床医学引用本文复制引用
魏献政,张书瑜,金钰航,王璞,王旭,郑骏年,刘丹,施明..国产与进口CD3/CD28激活磁珠制备CAR-T细胞的性能对比[J].中国肿瘤生物治疗杂志,2025,32(5):492-497,6.基金项目
国家自然科学基金(No.82304535) （No.82304535）
