中国肿瘤生物治疗杂志2025，Vol.32Issue(5)：498-509,12.DOI:10.3872/j.issn.1007-385x.2025.05.007
PKMYT1在胶质瘤中的表达及其与预后、药物敏感性和免疫浸润关联性的分析
Expression of PKMYT1 in glioma and its association with prognosis,drug sensitivity,and immune infiltration
摘要
Abstract
Objective:To analyze by bioinformatics the association between the expression of membrane-associated tyrosine/threonine protein kinase 1(PKMYT1)in glioma with its prognostic value,biological function,drug sensitivity,gene mutation,and immune infiltration.Methods:The differential expression of PKMYT1 was analyzed based on the Chinese Glioma Genome Atlas database(CGGA)and the Cancer Genome Atlas database(TCGA).Pathways likely to be enriched for PKMYT1 were predicted by gene ontology analysis(GO)and gene set enrichment analysis(GSEA).PKMYT1 was subjected to Pearson correlation and gene set variation analysis(GSVA)with cell cycle-related genes and gene sets.The survival prognosis,gene mutation,drug sensitivity and immune infiltration were further analyzed for PKMYT1 high and low expression groups of glioma patients.Results:PKMYT1 was significantly highly expressed in WHO high-grade glioma(P<0.000 1),IDH wild-type glioma(P<0.05),and glioblastoma(P<0.000 1).Overall survival(OS)of patients in the PKMYT1 low expression group was significantly higher than that of the high expression group(P<0.05).Cox regression analysis showed that PKMYT1 expression level was an independent prognostic factor for OS(P<0.05).GO and GSEA analyses showed that the gene sets co-expressed with PKMYT1 were mainly enriched in signaling pathways such as cell cycle,DNA replication and DNA damage repair.Pearson correlation and GSVA analyses showed that the expression of PKMYT1 was significantly and positively correlated with the cell cycle-related genes,gene sets and cell cycle checkpoint genes(P<0.01).Drug sensitivity analysis revealed that patients in the PKMYT1 high expression group had high sensitivity osimertinib,dabrafenib,carmustine and cediranib(P<0.05).Mutation analysis revealed that the IDH1 gene had a higher mutation frequency in the PKMYT1 low expression group.The results of immune infiltration analysis showed that PKMYT1 expression was significantly positively correlated with glioma stroma score(r=0.13,P<0.001),immune score(r=0.11,P<0.01)and ESTIMATE score(r=0.13,P<0.001);and was significantly positively correlated with immune cell infiltration level of regulatory T(Treg)cells and M2-type macrophages(P<0.05).Conclusion:Patients with high PKMYT1 expression have a poorer prognosis,and the mechanism may be related to tumor immune infiltration and cell cycle regulation.PKMYT1 is expected to be a potential target for the diagnosis and treatment of glioma.关键词
胶质瘤/膜相关酪氨酸/苏氨酸蛋白激酶1/细胞周期/预后/免疫浸润/药物敏感性Key words
glioma/membrane-associated tyrosine/threonine protein kinase 1(PKMYT1)/cell cycle/prognosis/immune infiltration/drug sensitivity分类
临床医学引用本文复制引用
解辉平,刘佳骏,陈佳彬,朱丽华,张志斐,杨兆勇..PKMYT1在胶质瘤中的表达及其与预后、药物敏感性和免疫浸润关联性的分析[J].中国肿瘤生物治疗杂志,2025,32(5):498-509,12.基金项目
河北省自然科学基金-中医药联合基金培育项目(No.H2022209048) （No.H2022209048）
