胺鲜酯浸种对NaCl胁迫下紫花苜蓿种子萌发及幼苗生长的影响OA北大核心
The effect on seed germination and seedling growth of soaking seeds with diethyl aminoethyl hexanoate in alfalfa under NaCl stress
紫花苜蓿种子的发芽过程受到多种因素的影响,其中盐分胁迫是一个关键的限制因素.尽管胺鲜酯(DA-6)作为一种新型的植物生长促进剂在农业生产中受到了广泛的关注和应用,但其在盐胁迫条件下对紫花苜蓿种子发芽的具体作用机制尚未有明确的研究报告.因此,本研究探究了 150 mmol·L-1NaCl溶液胁迫下 2.5、5.0、10.0、15.0 mmol·L-1的DA-6浸种对紫花苜蓿种子萌发及幼苗生长的影响.研究结果揭示,在高浓度NaCl环境中,紫花苜蓿种子的活力受到严重影响,其发芽过程也遭受明显的抑制.然而,通过使用不同浓度的DA-6进行种子浸泡处理,可以有效地提升种子的发芽率、发芽势、发芽指数,以及根长、茎长、鲜重和干重等指标,从而整体上促进种子的萌发过程.特别地,当DA-6的浓度达到2.5 mmol·L-1 时,对于提高NaCl胁迫条件下种子的抗氧化酶活性表现出显著效果.DA-6浸种通过提高可溶性蛋白、可溶性糖、游离脯氨酸的含量,从而提高紫花苜蓿对盐的耐受性,促进种子萌发.综合分析表明,2.5、5.0 mmol·L-1 DA-6对正常环境和NaCl胁迫下紫花苜蓿种子萌发及幼苗生长均有积极的影响.
The germination process in Medicago sativa seeds is influenced by various factors,among which salt stress is a key limiting factor.There is widespread interest in application of the emerging plant growth promoter diethyl aminoethyl hexanoate(DA-6)in agricultural production,but there is no clear research report on its specific mechanism of action on alfalfa seed germination under salt stress conditions.Consequently,this investigation examined the impact of a 150 mmol·L-1 NaCl environment on the development of alfalfa seeds when subjected to DA-6 concentrations of 2.5,5.0,10.0,and 15.0 mmol·L-1.Specifically,our analysis focused on the effects of DA-6 immersion on alfalfa seedlings at both the sprouting and the initial plant formation stages.The findings revealed a substantial decrease in the vitality of M.sativa seeds and a marked suppression of their ability to germinate under conditions of high NaCl concentration.Seed soaking treatment with DA-6 at various concentrations increased the germination rate,germination index,germination potential,root length,stem length,fresh weight and dry weight,and improved the seed germination.The 2.5 mmol·L-1 DA-6 seed soaking treatment increased the activity of antioxidant enzymes in seeds under salt stress.The practice of immersing alfalfa seeds in DA-6 has thus been shown to augment alfalfa seedling resistance to salinity and to stimulate seed sprouting.This enhancement is attributed to an upsurge in the levels of accessible proteins,sugars,and free proline within the seeds.Multivariate analysis showed that the 2.5 and 5.0 mmol·L-1 DA-6 concentrations had the most positive effect on alfalfa seed germination and seedling growth in an otherwise normal environment under NaCl stress.
崔灿;王梦琦;赵琬璐;刘新颖;鉴晶晶;严俊鑫
东北林业大学园林学院,黑龙江 哈尔滨 150000东北林业大学园林学院,黑龙江 哈尔滨 150000东北林业大学园林学院,黑龙江 哈尔滨 150000东北林业大学园林学院,黑龙江 哈尔滨 150000东北林业大学园林学院,黑龙江 哈尔滨 150000东北林业大学园林学院,黑龙江 哈尔滨 150000
紫花苜蓿胺鲜酯NaCl胁迫
Medicago sativadiethyl aminoethyl hexanoateNaCl stress
《草业学报》 2025 (6)
46-58,13
国家重点研发计划项目(2021YFD1500705,2021YFD1500600)资助.
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