森林与环境学报2025，Vol.45Issue(4)：347-357,11.DOI:10.13324/j.cnki.jfcf.202412046
石质山地不同植被恢复方式下的酶化学计量特征
Enzyme stoichiometric characteristics of four vegetation restoration methods in a rocky mountainous area
摘要
Abstract
To provide a scientific basis for soil nutrient management in rocky mountainous areas,this study investigated the effects of different vegetation restoration methods on soil enzyme stoichiometric characteristics and explored the limitations of soil microbial resources in rocky mountain plantations.The activities of β-glucosidase(BG),leucine aminopeptidase(LAP),N-acetyl-β-D-glucosidase(NAG),and alkaline phosphatase(ALP)in the 0-10 cm and 10-20 cm soil layers,along with soil properties and fine root characteristics of four plantations(Koelreuteria paniculata,Firmiana platanifolia,Pistacia chinensis,and Acer pictum)established in 2012 in a rocky mountainous area,were measured.Enzyme stoichiometric ratios of carbon to nitrogen,carbon to phosphorus,and nitrogen to phosphorus were calculated,and the effects of soil properties,fine root biomass and chemical composition on enzyme stoichiometric ratios were analyzed.The results showed that the activities of BG(P<0.05),NAG(P<0.01),LAP(P<0.01),and ALP(P<0.01)exerted differences among different vegetation restoration methods.In both soil layers,BG and ALP activities were significantly higher in the P.chinensis plantation than in the K.paniculata plantation(P<0.05).In the 0-10 cm soil layer,NAG and LAP activities were significantly higher in the K.paniculata plantation than in other plantations(P<0.05).Differences existed in the enzyme stoichiometric ratios among different vegetation restoration methods(P<0.01).In both soil layers,the carbon-to-nitrogen extracellular enzyme activity ratio was the smallest in the K.paniculata plantation,and the nitrogen-to-phosphorus extracellular enzyme activity ratio was the largest in the K.paniculata plantation.The carbon-to-phosphorus extracellular enzyme activity ratio in the 0-10 cm soil layer was the smallest in the A.pictum plantations and that in the 10-20 cm soil layer was the smallest in the F.platanifolia plantation.Only the carbon-to-phosphorus extracellular enzyme activity ratio did not show differences between soil layers.The redundancy analysis indicated that soil total nitrogen content,soil carbon-to-phosphorus ratio,total phosphorus content in fine roots,and total carbon content in fine roots were the main driving factors for the changes in enzyme stoichiometric ratios.In conclusion,the low total nitrogen content and high availability of phosphorus in the K.paniculata plantation led to a strong nitrogen limitation for soil microorganisms.The high available nitrogen content in soil and low total phosphorus content in fine roots of F.platanifolia,P.chinensis,and A.pictum plantations caused microbial limitations by carbon and phosphorus.关键词
酶化学计量比/微生物资源限制/石质山地/植被恢复/酶活性Key words
enzyme stoichiometric ratio/microbial resource limitation/rocky mountainous area/vegetation restoration/enzyme activity分类
林学引用本文复制引用
张琳婧,张宇恬,史珑燕,孟董悦,徐天懂,关庆伟..石质山地不同植被恢复方式下的酶化学计量特征[J].森林与环境学报,2025,45(4):347-357,11.基金项目
农业农村领域重大关键技术攻关项目"江苏典型困难立地高固碳树种筛选及碳增汇栽培关键技术研究与示范"(BE2022420). （BE2022420）
