辐射研究与辐射工艺学报2025，Vol.43Issue(3)：52-60,9.DOI:10.11889/j.1000-3436.2024-0114
60Co-γ射线辐照佛手提取液提升其生物活性
Enhancing the biological activity of Citri Sarcodactylis Fructus extract via 60Co-γ ray irradiation
摘要
Abstract
The extract of Citri Sarcodactylis Fructus was used as raw material and treated with 60Co-γ rays to enhance its polyphenols content.Its skin care effects,including moisturizing,skin irritation reduction,anti-oxidation,whitening,and anti-inflammatory properties,were evaluated.The results showed that the content of total polyphenols in the extract increased gradually with an increase in the adsorbed dose,and the scavenging efficiency of 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radicals was positively correlated with the adsorbed dose.The extract of Citri Sarcodactylis Fructus has a high moisturizing rate under the conditions of color-changing silica gel and 46%humidity,and it is better than that of glycerin and sodium hyaluronate.The results of skin irritation analysis showed that skin exhibited a fair and smooth state after the phenomenon of cuticle peeling at high concentrations,and there was no subsequent skin irritation at low concentrations.In terms of antioxidation,the total phenolic content and DPPH radical scavenging efficiency of the extracts were positively correlated with adsorbed dose in the range of 0-50 kGy irradiation.It showed a good inhibitory effect on the production of melanin and tyrosinase in B16-F10 cells stimulated by α-MSH.After treatment with 30 kGy,the production of melanin in B16-F10 cells decreased by 48.97%and the activity of intracellular tyrosinase decreased to 42.41%.Additionally,the extract showed a strong inhibitory effect on the production and expression of inflammatory factors in LPS-treated RAW264.7 cells.The inhibition rate of IL-1β was 13.67%due to 30 kGy irradiation.The inhibition rates of IL-6 and TNF-α by 40 kGy irradiation were 27.01%and 6.88%,respectively.The skin care effect and safety of the irradiated extract of Citri Sarcodactylis Fructus were enhanced,making it highly suitable for practical applications.关键词
佛手提取液/辐照/保湿/刺激性/美白/抗炎Key words
KEYEORDS Citri Sarcodactylis Fructus extract/Irradiation/Moisturizing/Irritating/Whitening/Anti-inflammatory分类
轻工纺织引用本文复制引用
先丽,倪茂君,张晓彬,胡椹,刘思阳,杜浩田,王静霞,彭朝荣..60Co-γ射线辐照佛手提取液提升其生物活性[J].辐射研究与辐射工艺学报,2025,43(3):52-60,9.基金项目
2024年四川省省级科研院所基本科研业务,四川省重点研发项目(2024YFNH0012) Supported by Basic Research of Sichuan Provincial Scientific Research Institutions in 2024 and Key R&D Project of Sichuan Province(2024YFNH0012) （2024YFNH0012）
