海南大学学报（自然科学版）2025，Vol.43Issue(2)：121-127,7.DOI:10.15886/j.cnki.hdxbzkb.2024070101
卵形鲳鲹IRF7的原核表达、纯化及其多克隆抗体制备
Prokaryotic expression and polyclonal antibody preparation of Trachinotus ovatus interferon regulatory factor IRF7
摘要
Abstract
Interferon regulatory factor 7(IRF7)plays important roles in immune regulation,antivirus,and antisepsis,cell proliferation and cell apoptosis,however,the role of IRFs in anti-disease response remains poorly understood.In the report,the recombinant Trachinotus ovatus IRF7(rTroIRF7)protein was prepared in Escherichia coli,purified,and used to produce polyclonal antibodies.The pET32a(+)/Escherichia coli BL21(DE3)expression system was used for the inducible expression of TroIRF7.Under the conditions,0.1 mmol·L-1 IPTG,24 h,20 ℃,the soluble recombinant protein rTroIRF7 was obtained.The molecular mass of the rTroIRF7 protein was 50 kDa.The rTroIRF7 protein was purified by nickel affinity chromatography.The purified fusion protein rTroIRF7 was used as an antigen to immunize mouse for the preparation of polyclonal antibody.ELISA was performed,and the results indicated that the total titer of the polyclonal antibody of rTroIRF7 was 1∶25 600.关键词
干扰素调节因子7/卵形鲳鲹/原核表达/多克隆抗体Key words
interferon regulatory factor 7/Trachinotus ovatus/prokaryotic expression/polyclonal antibody分类
农业科技引用本文复制引用
吴莹,周永灿,孙云..卵形鲳鲹IRF7的原核表达、纯化及其多克隆抗体制备[J].海南大学学报（自然科学版）,2025,43(2):121-127,7.基金项目
国家自然科学基金地区项目(42066007) （42066007）
