广东药科大学学报2025，Vol.41Issue(3)：88-95,8.DOI:10.16809/j.cnki.2096-3653.2025040701
不同产地冬瓜皮HPLC特征图谱及其黄酮类成分研究
Study on HPLC characterization and flavonoid components of winter melon peel from different habitats
摘要
Abstract
Objective To establish a high-performance liquid chromatography(HPLC)characteristic chromatogram method for winter melon peel medicinal materials,and simultaneously determine the contents of flavonoid components such as vitexin rhubarb and isovitexin-2″-O-rhubarb.Combined with the analysis data of cluster analysis(Cluster Analysis),principal component analysis(Principal Component Analysis),and orthogonal partial least squares discriminant analysis(Orthogonal Partial Least Squares Discriminant Analysis),an overall evaluation of winter melon peels from different origins was conducted.Methods A Dikma Platisil ODS(250 mm×4.6 mm,5 μm)chromatographic column was used.Acetonitrile(A)and 0.1%formic acid(B)were used as the mobile phases.Gradient elution was performed.The detection wavelength was 270 nm,the flow rate was 1.0 mL/min,and the column temperature was 35℃.Taking the flavonoid content and the similarity of common peaks in characteristic spectra as the main evaluation indicators,combined with the chemical model,the differences of winter melon peel medicinal materials from different origins were analyzed.Results The characteristic spectra of the 15 batches of winter melon peel samples all presented 5 common characteristic peaks,and the similarity with the control spectra was all greater than 0.900.Three components,namely 4-hydroxybenzoic acid,vitexin rhamnoside,and isovitexin-2″-O-rhamnoside,were identified,and the flavonoid components were quantitatively determined.Cluster analysis classified them into 4 categories,which was consistent with the results of PCA analysis.Through VIP values,the principal component substance vitrecin rhunoside was screened out as the differential substance affecting the classification.Orthogonal partial least squares discriminant analysis also further verified the above results.Conclusion The characteristic chromatograph(HPLC)and content determination method are stable and reliable,which can be used to evaluate the quality of winter melon peels from different origins,providing a scientific basis for the quality control of winter melon peel medicinal materials and the medicinal development of winter melon peels.关键词
冬瓜皮/化学模式/黄酮类/特征图谱Key words
winter melon peel/chemical model/flavonoids/characteristic chromatogram分类
医药卫生引用本文复制引用
蔡小兵,江斌,张辉..不同产地冬瓜皮HPLC特征图谱及其黄酮类成分研究[J].广东药科大学学报,2025,41(3):88-95,8.基金项目
深圳市科技计划项目(JSGG20191129093418578) （JSGG20191129093418578）
