湖北农业科学2025，Vol.64Issue(5)：25-32,48,9.DOI:10.14088/j.cnki.issn0439-8114.2025.05.005
耐硒菌株Bacillus cereus ZJ2的筛选及四种碳源对其合成纳米硒稳定性的影响
Isolation of selenium-resistant strain Bacillus cereus ZJ2 and the effects of four carbon sources on the stability of selenium nanoparticles synthesized by the strain
摘要
Abstract
To better utilize microorganisms for environmental bioremediation,reduce selenium pollution in soil,and rationally exploit selenium resources,a selenium-tolerant strain,ZJ2,was isolated from selenium-rich soil in Yutangba,Enshi City,Hubei Province.Molecular biological identification confirmed it as Bacillus cereus.This strain exhibited high tolerance and reduction capability to sodi-um selenite(Na2SeO3),converting it into stable biogenic selenium nanoparticles(SeNPs).Additionally,the effects of four different carbon sources on the sodium selenite reduction efficiency and the stability of synthesized SeNPs were investigated.The results showed that strain ZJ2 had a 92%reduction capacity to 5 mmol/L sodium selenite.The bio-nanoselenium synthesised by this strain was spheri-cal particles with an average particle size of(188.42±2.63)nm and a Zeta potential of(-41.17±1.77)mV.It was found that the addi-tion of rhamnose and glucose promoted the reduction of sodium selenite by strain ZJ2,with an increase in the reduction rate of 37.48%and 39.53%,respectively.In addition,rhamnose had a good promoting effect on the synthesis of stable selenium nanoparticles by the strain.Based on a comprehensive evaluation of the sodium selenite reduction capability,characterization of selenium nanoparticles(SeNPs)and their stability,it was demonstrated that the addition of rhamnose significantly enhanced the ability of Bacillus cereus ZJ2 to efficiently synthesize more stable biogenic SeNPs.关键词
耐硒菌株/蜡样芽孢杆菌(Bacillus cereus)/纳米硒/还原率/稳定性/碳源Key words
selenium-resistant strain/Bacillus cereus/nano-selenium/reduction rate/stability/carbon source分类
农业科技引用本文复制引用
何嘉丽,刘梦绮,钟文怡,何毅,王璋倩,程水源..耐硒菌株Bacillus cereus ZJ2的筛选及四种碳源对其合成纳米硒稳定性的影响[J].湖北农业科学,2025,64(5):25-32,48,9.基金项目
湖北省自然科学基金项目(2023AFB968) （2023AFB968）
湖北省自然科学基金杰出青年项目(2023AFA102) （2023AFA102）
湖北省工业微生物重点实验室项目(2024KF07) （2024KF07）
武汉轻工大学科研创新计划项目(2019y05) （2019y05）
武汉轻工大学硒科学与工程和恩施德源硒材料工程科技有限公司技术储备项目(Se2-202301) （Se2-202301）
