光学精密工程2025，Vol.33Issue(8)：1228-1237,10.DOI:10.37188/OPE.20253308.1228
靶向药物浓度筛选微流控器件的设计与制作
Design and fabrication of microfluidic device used for concentration screening of targeted-drugs
摘要
Abstract
To screen the concentrations of targeted drugs,a cell co-culture microfluidic device with an inte-grated concentration gradient generator was designed and fabricated.The principles of concentration gradi-ent generation,the concentration fields,the velocity fields,and the ability of cell co-culture within the de-vice were studied.A microchannel model capable of generating a 5∶1∶0 concentration gradient was de-signed based on the equivalent circuit principle,using a series-parallel structure to integrate the concentra-tion gradient unit with the cell co-culture unit.The velocity and concentration fields were simulated and validated using the laminar flow and mass transfer modules in the finite element simulation software Com-sol.The microfluidic device was fabricated using soft lithography techniques,and fluorescein sodium solu-tion and polystyrene suspension with a 1 µm diameter were used to simulate drug concentration distribu-tion.Co-culture of Hela cells and A549 cells was performed within the device,and the growth curves and folate receptor targeting of both cell types were analyzed.Simulation and experimental results indicate that the designed microfluidic device could generate the desired concentration gradient(5∶1∶0 ratio),with con-centration variation within each branch cell culture unit below 0.1%.The velocity distribution in the cell culture unit is uniform,with an average flow velocity of(7.1±0.3)µm/s under an inlet flow rate of 925 µm/s.The growth of both cell types follows an S-shaped growth curve,and membrane staining experi-ments demonstrate the successful parallel co-culture of two cell types within the microfluidic device.These findings demonstrate that microfluidic device could simulate the in vivo fluidic environment of cells and provide a technological platform for targeted drug concentration screening and personalized therapy.关键词
微流控/细胞微环境/靶向药物浓度筛选/平行共培养Key words
microfluidics/cell microenvironment/targeted drugs concentration screening/parallel co-culture分类
信息技术与安全科学引用本文复制引用
霍兆林,丁来钱,亓东锋,刘冲,魏娟..靶向药物浓度筛选微流控器件的设计与制作[J].光学精密工程,2025,33(8):1228-1237,10.基金项目
国家重点研发计划项目(No.2022YFB4600402) （No.2022YFB4600402）
山东省自然科学青年基金项目(No.ZR2021QE130) （No.ZR2021QE130）
山东省青年泰山学者项目(No.tsqn202306192) （No.tsqn202306192）
