河南农业科学2025，Vol.54Issue(6)：121-128,8.DOI:10.15933/j.cnki.1004-3268.2025.06.013
秋水仙素诱导香菜四倍体的研究
Study on the Induction of Tetraploid in Coriander by Colchicine
摘要
Abstract
Using Australian four season large leaf coriander seeds as experimental materials,different mass fractions of colchicine(0.1%,0.2%,0.4%,0.6%,0.8%)and different time periods(2,4,6,8,10 h)were used for mutagenesis treatment to study the effect of colchicine on the induction of tetraploid coriander.The results showed that with the increase of colchicine concentration and the prolongation of treatment time,the germination rate and seedling rate of coriander seeds showed a significant downward trend.Among them,0.4%colchicine treatment for 2 h was the optimal induction condition,with the highest germination rate,seedling rate,and induction rate of 80%,60%,and 18.7%,respectively.The physiological indicators of coriander seedlings significantly improved,with chlorophyll a+b,soluble protein,and soluble sugar contents of 1.49,9.87,and 17.54 mg/g,respectively,which increased by 88.61%,58.97%,and 53.88%compared to the control.Through cytological identification,it was found that compared with diploid plants,tetraploid plants showed a significant reduction in the number of stomata per unit area of their leaves,an increase in stomata,and a doubling of the chromosome number in root tip cells(4n=44).In addition,tetraploid coriander plants also exhibited excellent traits such as wide and thick green leaves,thicker and longer purple stems,strong aroma,and larger flowers and inflorescences.关键词
香菜/秋水仙素/染色体加倍/四倍体诱导Key words
Coriander/Colchicine/Chromosome doubling/Tetraploid induction分类
农业科技引用本文复制引用
凌娜,张少京,单思祺,史本林,潘一展,孟凡荣..秋水仙素诱导香菜四倍体的研究[J].河南农业科学,2025,54(6):121-128,8.基金项目
河南省高等学校青年骨干教师培养计划资助项目(2023GGJS188) （2023GGJS188）
河南省科技攻关项目(252102110156) （252102110156）
2024年度河南省高等教育教学改革研究与实践项目(2024SJGLX0587,2024SJGLX0588) （2024SJGLX0587,2024SJGLX0588）
河南省自然科学基金项目(242102110295) （242102110295）
