贵州农业科学2025，Vol.53Issue(6)：72-80,9.DOI:10.3969/j.issn.1001-3601.2025.06.008
基于UPLC-MS/MS分析贵州3个主栽茶树鲜叶氨基酸及其代谢物含量
Amino Acids and Metabolite Contents in Fresh Leaves of Three Main Tea Cultivars from Guizhou Based on UPLC-MS/MS
摘要
Abstract
[Objective]The differences in amino acid and metabolite content of fresh leaves of three main tea cultivars in Guizhou Province,including Camellia sinensis cv.Qiancha 1(QC1),Camellia sinensis cv.Qianmei 601(QM601)and Camellia sinensis cv.Fuding Daba(FDDB),were clarified,which provided theoretical basis for processing technologies of developing cultivar-specific tea and high-value resource utilization.[Method]Fresh leaves(one bud and one leaf)of QC1,QM601 and FDDB were used as the study materials.Ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to quantify amino acid and metabolite content and screen key differential metabolites.[Result]68 amino acids and metabolites were detected from the three cultivars,including 19 protein amino acids,16 non-protein amino acids and 33 amino acid metabolites.QM601 had the highest total protein amino acid content at 82 443.78 μg/g and was significantly higher than FDDB and QC1,with notably high levels of glutamic acid(34 144.06 μg/g),glutamine(43 116 μg/g)and arginine(1 766.74 μg/g).FDDB and QC1 had higher γ-aminobutyric acid(GABA)content(2 794.77 μg/g and 3 272.98 μg/g)than QM601(690.30 μg/g).Cluster analysis showed that QM601 formed a separate cluster,while FDDB and QC1 clustered together,reflecting metabolic differentiation between large-leaf and medium-small-leaf cultivars.QM601 had 44 and 45 significant differential metabolites compared to FDDB and QC1,respectively,with 20 common differential metabolites.[Conclusion]The high protein amino acid content of QM601 provides a material basis for enhancing flavor and reducing bitterness in tea processing.FDDB and QC1,being rich in GABA,show potential for developing functional tea products.关键词
茶树/福鼎大白/黔茶1号/黔湄601/UPLC-MS/MS/氨基酸/氨基酸代谢物Key words
tea plant/Camellia sinensis cv.Fuding Daba/Camellia sinensis cv.Qiancha 1/Camellia sinensis cv.Qianmei 601/UPLC-MS/MS/amino acids/amino acid metabolites分类
农业科技引用本文复制引用
方仕茂,代新龙,罗金龙,戴宇樵,刘忠英,潘科,沈强..基于UPLC-MS/MS分析贵州3个主栽茶树鲜叶氨基酸及其代谢物含量[J].贵州农业科学,2025,53(6):72-80,9.基金项目
贵州省科技计划项目"黔茶 1 号芳樟醇高积累的分子机理研究"(黔科合基础-ZK[2023]一般 165) （黔科合基础-ZK[2023]一般 165）
2024年度中央引导地方科技发展资金项目"贵州省食用菌与茶产业循环经济技术成果转化"(黔科合中引地[2024]026-5) （黔科合中引地[2024]026-5）
贵州省农业科学院 2025年度关键核心技术项目"贵州主要特色农产品数字化拼配关键技术研究"(黔农科GJHX[2025]06号) （黔农科GJHX[2025]06号）
贵州省农业科学院青年基金项目"基于代谢组学的黔茶1号白茶加工工艺及品质形成机理研究"(黔农科一般基金[2024]13) （黔农科一般基金[2024]13）
贵州省农业科学院 2023年省财政科研专项资金项目"基于黔茶系列品种的贵州特色茶叶产业加工技术体系构建"(黔农科科技创新[2023]12号) （黔农科科技创新[2023]12号）
