甘蔗糖业2025，Vol.54Issue(3)：19-32,14.DOI:10.3969/j.issn.1005-9695.2025.03.003
瑞丽割手密光合气体交换参数特性的差异分析
Differential Analysis of Photosynthetic Gas Exchange Parameters Characteristics of Ruili Saccharum spontaneum
摘要
Abstract
In order to understand the genetic variation of photosynthetic gas exchange parameters of Yunnan Saccharum spontaneum clones,and to screen the high luminous efficiency excellent germplasm resources,and lay the germplasm foundation for the breeding of high luminous efficiency varieties.Six photosynthetic gas exchange parameters,including net light rate(A),stomatal conductance(Gs),intercellular CO2 concentration(Ci),transpiration rate(E),transpiration efficiency(TE)and instantaneous water use efficiency(WUE),were determined by LCpro T photosynthesis analyzer at the large growth stage of 14 Ruili S.spontaneum clones.The variation characteristics of gas exchange parameters were clarified by variance analysis,generalized heritability calculation,correlation and principal component analysis,and the high-quality germplasm was screened by cluster analysis and discriminant analysis.The results showed that the 6 photosynthetic gas exchange parameters had significant or extremely significant differences among the 14 samples,and the variation was as follows:Gs＞Ci＞WUE＞TE＞E＞A.The generalized heritability of all parameters was greater than 30%,and the maximum generalized heritability of Ci was 73.505%.The net light rate(A)was positively correlated with stomatal conductance(Gs)and instantaneous water use efficiency(WUE),positively correlated with intercellular CO2 concentration(Ci),and negatively correlated with transpiration efficiency(TE).The principal component analysis synthesized six photosynthetic gas exchange parameters into two common factors,"carbon assimilation factor"and"water use efficiency",whose cumulative contribution rate reached 82.753%.The first common factor,"carbon assimilation factor",was the more important factor,whose contribution rate reached 54.441%.By cluster analysis,14 Ruili Saccharum spontaneum germlines were divided into 3 categories at the Euclidean distance of 4.50,and the results were consistent with the principal component score.Among them,the 6 photosynthetic gas indexes of 7 samples of Group Ⅰ,including 2023RLL-4,2023RLL-8,2023RLL-9,2023RLL-10,2023RLL-12,2023RLL-13 and 2023RLL-14,were excellent.Among them,2023RLL-9,2023RLL-10 and 2023RLL-12 had higher principal component scores,which should be paid more attention,and their utilization should be strengthened in the breeding process of high luminous efficiency.关键词
甘蔗/割手密/高光效/气体交换参数/遗传差异Key words
Sugarcane/Saccharum spontaneum L./High luminous efficiency/Gas exchange parameter/Genetic variation分类
农业科技引用本文复制引用
俞华先,桃联安,安汝东,刘家勇,昝逢刚,赵丽萍,赵勇,艾静..瑞丽割手密光合气体交换参数特性的差异分析[J].甘蔗糖业,2025,54(3):19-32,14.基金项目
国家重点研发计划项目(2022YFD2301100) （2022YFD2301100）
广西科技重大专项(桂科AA23073001) （桂科AA23073001）
国家基金委(地区)科学基金(31860406、31960448) （地区）
