经济动物学报2025，Vol.29Issue(3)：220-224,5.DOI:10.13326/j.jea.2025.1829
基于PCR技术的CHD基因检测法与传统形态学方法在鸽性别鉴定中的比较
Comparative Analysis of CHD Gene PCR Amplification and Conventional Morphological Approaches for Avian Sex Determination in Pigeons
摘要
Abstract
Rapid and accurate identification of pigeon sex is one of the most important tasks to increase pigeon breeding rate and economic benefit.Five traditional methods were used to identify the sex of 100 4-month-old pigeons.At the same time,molecular biology techniques were used,including chromobox helicase DNA binding gene(CHD gene)specific primers for PCR amplification,sequencing and nucleotide homology analysis.And the comparison between the traditional identification methods and PCR method was conducted.The results showed that in traditional avian sexing methods,the accuracy rates were determined as follows:vent sexing demonstrated 57%accuracy,vocalization analysis achieved 52%,skeletal morphology assessment reached 48%,plumage characteristics evaluation attained 45%,and morphometric analysis(body size evaluation)showed 47%accuracy.Notably,the integrated approach combining vent sexing,vocalization analysis,and skeletal morphology assessment yielded the highest diagnostic efficacy with an 86%accuracy rate,the PCR method could improve the accuracy of sex identification of pigeon rapidly and accurately compared with the traditional methods.This experiment provides an accurate method for sex identification of pigeons and plays an important role in improving the economic benefits for pigeon farm.关键词
鸽子/性别鉴定/CHD基因/PCR技术/传统方法Key words
pigeons/sex identification/CHD gene/PCR/traditional methods分类
农业科技引用本文复制引用
陈红强,宋欣雨,张海洋,杨浩,阿合朱力·加库,胡建军..基于PCR技术的CHD基因检测法与传统形态学方法在鸽性别鉴定中的比较[J].经济动物学报,2025,29(3):220-224,5.基金项目
兵团科技局科技特派员创新创业项目(2022CB024),大学生创新创业训练计划项目(2023011),兵团"三区"科技人才专项团队项目(BT1720220042G) （2022CB024）
