经济动物学报2025，Vol.29Issue(3)：225-233,9.DOI:10.13326/j.jea.2025.1990
狄氏副拟杆菌改善ETEC诱导小鼠腹泻的作用机制
Mechanism of Parabacteroides distasonis in Ameliorating ETEC-Induced Diarrhea in Mice
摘要
Abstract
Enterotoxigenic Escherichia coli(ETEC)is a major causative agent of diarrhea for neonatal livestock.Prolonged antibiotic use for treatment risks inducing bacterial resistance,so probiotics are preferred strategy for preventing and treating diarrhea in newborn animals.This study aimed to establish an ETEC-induced murine diarrhea model and investigate the therapeutic effects of Parabacteroides distasonis(Pd)using fluorescence quantitative PCR,Western blotting,ELISA,and immunofluorescence.Results demonstrated that Pd significantly reduced serum levels of pro-inflammatory cytokines(interleukin-8,C-reactive protein,and tumor necrosis factor-α)and suppressed their expression in intestinal tissues of ETEC-challenged mice.Furthermore,Pd upregulated both gene and protein expression of intestinal tight junction markers(Occludin,Claudin-1,and Zo-1)in ETEC-infected mice.These findings indicate that Pd alleviates ETEC-induced diarrhea by attenuating intestinal inflammation and enhancing intestinal barrier integrity,providing a theoretical basis for its application in preventing and treating neonatal livestock diarrhea.关键词
狄氏副拟杆菌/产肠毒素大肠杆菌/腹泻/紧密连接蛋白Key words
Parabacteroides distasonis/Enterotoxigenic Escherichia coli/diarrhea/tight junction protein分类
畜牧业引用本文复制引用
王佳,郭佳楠,宫金烁,杨文杰,张磊,石春卫,陈宏亮,张迪..狄氏副拟杆菌改善ETEC诱导小鼠腹泻的作用机制[J].经济动物学报,2025,29(3):225-233,9.基金项目
吉林省科技发展计划项目(20220202056NC),国家自然科学基金项目(32373095) （20220202056NC）
