菌物研究2025，Vol.23Issue(3)：211-222,12.DOI:10.13341/j.jfr.2023.1612
高效腐解玉米秸秆耐低温真菌菌株的筛选及评价
Screening and Evaluation of Fungal Strains for Efficient Decomposition of Corn Straws at Low Temperature
摘要
Abstract
Forty-five fungal strains were screened to show the activity of laccase,cellulase,hemicellulose or peroxidase by a plate rapid screening method combined with fermentation culture at low temperatures.Within these strains,the high enzyme activity reached up to 7.47 U/mL(L2431)and 7.2 U/mL(X1957)for laccase,239 U/mL(L743)and 167 U/mL(L1675)for cellulase,179.3 U/mL(YF410),120.0 U/mL(H382)and 113.3 U/mL(L2431-3)for hemicellulose,and 25.1 U/mL(L2431),24.9 U/mL(2423)and 15.9 U/mL(L2419)for peroxidase respectively under the liquid fermentation cultured at 20 ℃ in 10 days.After that,six strains were selected furthermore for the decomposition test of corn straws at low temperatures.Enzyme activities and degradation rates of corn straw components were measured in 10,20,30,40 and 50 days,respectively,indicating that the time needed by the enzymes to reveal their maximum activities at the low temperature varied among strains.Although there existed significant differences in the degradation rates for each component of corn straws among the above strains,the total degradation rates of corn straws increased as time proceeded for all the strains.The degradation rates of lignin,cellulose and hemicellulose in corn straws were 18.2％-25.1％,24.1％-44.6％and 19.3％-26.3％,respectively while the total degradation rates of corn straws were 61.8％-87.0％in 50 days.The above results demonstrated clearly that the fungal strains screened at the present study were able to adapt to the low-temperature environments without the loss of their high enzymatic activity and high efficiency in decomposition of corn straws,providing strong and positive evidence for future promotion and application of straw returning measures in the cold areas of northeast China.关键词
真菌菌株/平板筛选/木质纤维素酶/玉米秸秆/降解Key words
fungal strain/plate screening/lignocellulolytic enzymes/corn straw/decomposition分类
生物学引用本文复制引用
杨会敏,李伟,汪世华,尹文兵..高效腐解玉米秸秆耐低温真菌菌株的筛选及评价[J].菌物研究,2025,23(3):211-222,12.基金项目
中国科学院A类战略性先导科技专项(XDA28030400) （XDA28030400）
