菌物研究2025，Vol.23Issue(3)：223-230,8.DOI:10.13341/j.jfr.2023.1660
常压低温等离子体对羊肚菌栽培基质细菌群落的影响
Effect of Low-Temperature Plasma on Bacterial Community of Morchella Cultivation Substrate
摘要
Abstract
Cultivation substrate of Agaricus Morchella was used to investigate the sterilization effect of bacteria through the low-temperature plasma technology carried out at atmospheric pressure.High-throughput sequencing technology was performed to analyze the changes of bacterial community,such as composition and diversity in the substrate,before and after treatments of low-temperature and room-temperature as well as low-temperature plasma.Our results showed that the diversity,richness and evenness of bacterial community were significantly reduced after treatment by low-temperature plasma,with the largest decrement rate up to 32％,34％and 9％,respectively.Among different families detected from the bacterial community,however,relative richness of Burkholderiaceae increased continuously after treatment while among the genera,the dominant bacterium was Aquabacterium after treatment.The bactericidal rate of low-temperature plasma treatment against a variety of pathogenic bacteria such as Streptomyces,Xanthomonas,Ochrobactrum,and Paraburkholderia was above 99％.This study,as the first time,applied the new technology by using the atmospheric low temperature plasma for substrate sterilization of Morchella,which could effectively kill a variety of pathogenic bacteria,thereafter,demonstrating a good example and prospects for the future applications of above technology to the effective sterilization and preservation of Morchella cultivation substrates.关键词
常压低温等离子体/羊肚菌栽培基质/细菌多样性/高通量测序Key words
atmospheric low-temperature plasma/Morchella culture substrate/bacterial diversity/high-throughput sequencing分类
园艺学与植物营养学引用本文复制引用
贺雪峰,樊灏,周桂灵,杨柳,王秀茹,王涛,沈振兴..常压低温等离子体对羊肚菌栽培基质细菌群落的影响[J].菌物研究,2025,23(3):223-230,8.基金项目
西安市科学技术局农业技术研发项目(21NYYF0041) （21NYYF0041）
