江苏农业学报2025，Vol.41Issue(5)：833-839,7.DOI:10.3969/j.issn.1000-4440.2025.05.001
小麦γ-醇溶蛋白基因Tagli-γ-11的克隆及其互作蛋白质分析
Cloning of γ-gliadin gene Tagli-γ-11 and interactive proteins analysis in wheat
摘要
Abstract
To further analyze the regulation mechanism of γ-gliadin gene in flour quality of wheat,the Tagli-γ-11 gene was successfully cloned from Zhengmai 158(low protein content and high dough strength).Bioinformatic analysis re-vealed that this gene possessed the typical structural characteristics of γ-gliadin,containing eight conserved cysteine residues.A celiac disease(CD)epitope(PQQSFPQQQ)was identified within repeat domainⅡof the Tagli-γ-11 protein,located at amino acid positions 133-141.Using the yeast two-hybrid(Y2H)system,the cDNA library of Zhengmai 158 was screened,yielding eight candidate proteins poten-tially interacting with Tagli-γ-11.These included cysteine protease,fructose-bisphosphate aldolase,cysteine-rich and transmembrane domain-containing protein 1,(1,3∶1,4)-β-D-glucanase,auxin response factor 17-like(ARF17-like),cell number regulator 8-like(CNR8-like),ubiquitin-associated domain-containing protein DSK2b,transcription factor PIF1-like.The rotation validation assays performed on cysteine-rich and transmembrane do-main-containing protein 1,fructose-bisphosphate aldolase,and(1,3∶1,4)-β-D-glucanase confirmed physical interaction with Tagli-γ-11.Based on these results,it was hypothesized that Tagli-γ-11 interacted with these proteins and participated in the synthesis and degradation of storage proteins(such as gliadin)and starch within wheat grains,as well as in repro-ductive growth processes.关键词
小麦/Tagli-γ-11基因/酵母双杂交/回转验证Key words
Triticum aestivum L./Tagli-γ-11 gene/yeast two-hybrid system/rotation validation分类
农业科技引用本文复制引用
王沙沙,何宁,汪庆昌,黄超,宋晓,晁岳恩..小麦γ-醇溶蛋白基因Tagli-γ-11的克隆及其互作蛋白质分析[J].江苏农业学报,2025,41(5):833-839,7.基金项目
河南省科技攻关项目(232102110220) （232102110220）
河南省农业科学院科技攻关项目 （）
河南省农业科学院自主创新项目(2024ZC001) （2024ZC001）
