菌物研究2025，Vol.23Issue(3)：231-239,9.DOI:10.13341/j.jfr.2024.1841
基于代谢组和蛋白组联合分析栽培基质对银耳氨基酸合成的影响
Integrated Study of Metabolomics and Proteomics Reveals Substrate-driven Regulation of Amino Acid Biosynthesis in Tremella fuciformis
摘要
Abstract
In order to explore the regulatory effect of culture substrates on amino acid synthesis in the fruiting body of Tremella fuciformis,metabolomics and proteomics were applied in this study.Two kinds of substrates(a mixed substrate made of wheat bran and cottonseed hull and a Cyclobalanopsis substrate)were used to for the investigation,which revealed a result that the total contents of proteins and amino acids of T.fuciformis grown on the mixed substrate were significantly higher than those grown on the Cyclobalanopsis substrate.A total of 488 metabolites with significant differences in quantity between the two substrates were identified by LC-MS/MS,in which amino acids and peptides including their similar substances accounted for the highest proportion.Moreover,a total of 1 685 proteins were identified by using proteomics and 668 differentially expressed proteins were screened out.Correlation analysis of metabolomic and proteomic data revealed a presence of the regulatory network on the amino acid synthesis pathway,such as tryptophan,tyrosine and lysine and pentose phosphate pathways.Our study demonstrated,furthermore,an increment of NADPH content in the T.fuciformis grown on the mixed substrate,which led to the higher amino acid content of T.fuciformis than that of Cyclobalanopsis substrate.The above results obtained at the present study should help to understand the regulatory mechanism of amino acid synthesis under the different cultivation substrates in T.fuciformis and provide a theoretical basis for developing a high-quality and efficient cultivation model as well.关键词
银耳/代谢组学/蛋白质组学/氨基酸合成/NADPH路径Key words
Tremella fuciformis/metabolomics/proteomics/amino acid synthesis/NADPH pathway分类
生物学引用本文复制引用
许瀛引,张仕林,罗定平,张谦,舒雪琴,彭卫红,王勇..基于代谢组和蛋白组联合分析栽培基质对银耳氨基酸合成的影响[J].菌物研究,2025,23(3):231-239,9.基金项目
国家自然科学基金项目(32402653),通江县食用菌科研合作项目(SCTJ-2023001),四川省农科院重点科研项目(1+9KJGG007) （32402653）
