菌物研究2025，Vol.23Issue(3)：240-246,7.DOI:10.13341/j.jfr.2022.1520
褐环乳牛肝菌多糖提取工艺优化及结构表征和抗氧化活性
Optimization of Extraction Process and Analysis of Structures and Antioxidant Activity of Polysaccharide in Suillus luteus
摘要
Abstract
Suillus luteus,a rare wild fungus used as a source of both the edible food and Chinese medicines,has an important economic and medicinal value.In this study,single factor test combining with the use of response surface methodology was carried out to optimize the extraction process of polysaccharide in S.luteus.HPAEC and FTIR were used furthermore to analyze the structure of purified polysaccharide substances.Antioxidant activity of the polysaccharides in vitro,moreover,was determined by commercially available analysis kits.Optimal conditions for the extraction of polysaccharide were demonstrated as follows;an extraction time of 168.83 min,a temperature of 68.97 ℃ and a ratio of 45.1∶1.0 between the liquid volume(mL)and material weight(g),in which extraction rate of polysaccharide reached the maximum value of 6.24％.The polysaccharide detected in S.luteus was mainly composed of heteropolysaccharides,consisting of nine monosaccharides where glucose accounted for the largest proportion,and had a strong ability to scavenge free radicals in vitro in dependence upon doses.The above results obtained from this study should provide a basis of research for the development and utilization of S.luteus in future.关键词
褐环乳牛肝菌/多糖/响应面分析/结构分析/自由基清除Key words
Suillus luteus/polysaccharide/response surface analysis/structural analysis/free radi-cal scavenging分类
生物学引用本文复制引用
彭世超,杨淑蕊,张曌,赵峻嫡,戴映笛,邹怀豪,赵新玉,刘洋..褐环乳牛肝菌多糖提取工艺优化及结构表征和抗氧化活性[J].菌物研究,2025,23(3):240-246,7.基金项目
国家大学生创新创业训练计划项目(CXXM010),吉林省教育厅科学技术研究项目(JJKH20210328KJ) （CXXM010）
