江苏农业学报2025，Vol.41Issue(5)：848-857,10.DOI:10.3969/j.issn.1000-4440.2025.05.003
基于转录组解析玉米苗期根系对非生物胁迫的响应
Transcriptome-based analysis of the response of maize root to abiotic stress at seedling stage
摘要
Abstract
Abiotic stresses(drought,salt)have become one of the key factors restricting the yield and quality of maize.To elucidate the mechanisms of maize responsed to abiotic stresses,in this study,B73 was used as the experimental material,and four treatments were set up:control(CK),drought stress treatment(D),salt stress treatment(S)and drought+salt com-pound stress treatment(DS).Primary root length was dynamically monitored,and the root transcriptome was sequenced.The re-sults of phenotypic analysis showed that the primary root length was significantly shorter under different treatments compared with the control.A total of 1 526 differentially expressed genes were identified in the comparison groups between the control and differ-ent stress treatments.A total of 207,170 and 1 274 differentially expressed genes were identified in the comparison groups be-tween the control and drought stress,salt stress and drought+salt compound stress.Functional enrichment results suggested that these differentially expressed genes were mainly involved in the biological processes mediated by abscisic acid synthesis and sig-nal transduction,and transcription factors in response to abiotic stress.MYB and ERF family transcription factor genes showed the greatest response to abiotic stress.The results of this study lay a foundation for further revealing the molecular mechanism of maize seedlings in response to abiotic stress.关键词
玉米/非生物胁迫/转录组学/差异表达基因/脱落酸Key words
maize/abiotic stress/transcripto-mics/differentially expressed genes/abscisic acid分类
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段娅敏,王后苗,杨泽峰,徐辰武,李鹏程,王芸芸..基于转录组解析玉米苗期根系对非生物胁迫的响应[J].江苏农业学报,2025,41(5):848-857,10.基金项目
国家自然科学基金项目(32302654) （32302654）
