菌物研究2025，Vol.23Issue(3)：247-253,7.DOI:10.13341/j.jfr.2023.1647
MNP分子标记在食用菌品种精准鉴定中的应用与前景分析
Application and Prospects of MNP Molecular Markers for Precise Identification of Edible Mushrooms
摘要
Abstract
Precise identification of edible mushroom strains plays a crucial role in its production and germplasm innovation.In China,the general existence of close genetic relationships and severe homogeneity among different cultivars of commercial mushrooms is considered a serious problem.Current methods used for identifying edible mushroom strains are almost labor-intensive and lack accuracy and reproducibility as well.Therefore,development and application of advanced technology for the precise identification of edible mushrooms still remains as a challenging issue in their relevant industry.Multiple nucleotide polymorphism(MNP)molecular markers can be used for simultaneous detection of multiple sequence variations to provide high-resolution genetic information,which enables to distinguish the differences between edible mushrooms more precisely with high repeatability and accuracy.This article aims to investigate the advantages of use of MNP molecular markers for strain identification of edible mushrooms together with the review of their first application to the precise identification of Lentinula edodes and Flammulina filiformis carried out from the year 2022 to 2023.Our analysis of variation patterns of MNP markers demonstrated that not only precise identification but also traceability of mushroom strains could be gained,indicating the presence of a huge potential for their application.It is believed that through further research development,especially in combination with the phenotypic data,use of MNP molecular markers will continuously provide us with much more supports and solutions for the essential protection of edible mushroom germplasm resources,the breeding improvement as well as the quality control of industrial products.关键词
MNP分子标记/食用菌/谱系/精准鉴定Key words
MNP molecular marker/edible mushrooms/pedigree/precise identification分类
园艺学与植物营养学引用本文复制引用
刘飞,张明晢,曹槟,凌云燕,赵瑞琳..MNP分子标记在食用菌品种精准鉴定中的应用与前景分析[J].菌物研究,2025,23(3):247-253,7.基金项目
"十四五"国家重点研发计划项目(2022YFD1200605),现代农业产业技术体系北京市食用菌创新团队项目(BAIC03-01),河南省重点研发专项项目(221111110600) （2022YFD1200605）
