河南农业科学2025，Vol.54Issue(6)：11-20,10.DOI:10.15933/j.cnki.1004-3268.2025.06.002
苗期油莎豆在盐胁迫下的生理特性及转录组学分析
Physiological Characteristics and Transcriptomic Analysis of Cyperus esculentus L.at Seedling Stage under Salt Stress
摘要
Abstract
By using NaCl solution to simulate salt stress,the effects of 200 mmol/L NaCl on the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),as well as the contents of soluble protein(SP),proline(Pro)and malondialdehyde(MDA)in the leaves of Cyperus esculentus L.with Jisha No.2 as material,and the differentially expressed genes(DEGs)through transcriptome sequencing were analyzed,GO and KEGG pathway enrichment analysis of DEGs were conducted,and real-time fluorescence quantitative PCR(qRT-PCR)was used to verify the genes in the enrichment pathways,so as to preliminarily understand the salt tolerance mechanism of Cyperus esculentus L..The results showed that with the extension of salt stress time,the activities of SOD,POD and CAT first increased and then decreased,and reached the maximum on the sixth day;The contents of MDA,Pro and SP increased.Transcriptome sequencing was performed with the leaves of Cyperus esculentus L.treated with salt for 6 d,and 869 up-regulated DEGs and 972 down-regulated DEGs were detected.For GO enrichment analysis,DEGs were mainly enriched in cellular processes and metabolic processes in biological processes,cellular anatomical entity and intracellular in cellular components,and binding and catalytic activities in molecular functions;For KEGG enrichment analysis,DEGs were mainly enriched in pathways such as plant hormone signal transduction,plant-pathogen interaction,starch and sucrose metabolism,etc.qRT-PCR analysis showed that the expression levels of TRINITY_DN10189_c0_g1,TRINITY_DN2983_c0_g1,TRINITY_DN4981_c0_g1,TRINITY_DN1242_c0_g1,TRINITY_DN7378_c0_g1 and TRINITY_DN2888_c0_g1 increased,and the expression levels of TRINITY_DN6117_c0_g1,TRINITY_DN7833_c0_g1,TRINITY_DN8066_c0_g1,and TRINITY_DN8212_c0_g1 decreased,which were similar to the result of RNA-Seq,verifying the accuracy of the transcriptome result.关键词
油莎豆/盐胁迫/生理特性/转录组/GO/KEGGKey words
Cyperus esculentus L./Salt stress/Physiological characteristics/Transcriptome/GO/KEGG分类
农业科技引用本文复制引用
龙威,王靓,金羽琨,刘佳遥,魏尊苗,程艳,牟忠生..苗期油莎豆在盐胁迫下的生理特性及转录组学分析[J].河南农业科学,2025,54(6):11-20,10.基金项目
吉林省农业科技创新工程项目(CXGC2023RCG018,CXGC2022RCY025) （CXGC2023RCG018,CXGC2022RCY025）
吉林省创新平台(基地)和人才专项(20220508108RC) （基地）
