河南农业科学2025，Vol.54Issue(6)：100-109,10.DOI:10.15933/j.cnki.1004-3268.2025.06.011
草莓灰霉病菌拮抗内生细菌的分离鉴定及其抑菌作用
Isolation and Identification of Antagonistic Endophytic Bacteria against Strawberry Gray Mold and Their Antibacterial Effects
摘要
Abstract
In order to screen antagonistic strains of strawberry gray mold pathogens and provide strain resources for the biological control of strawberry gray mold,the endophytic bacteria with inhibitory effect on strawberry Botrytis cinerea were isolated and screened from healthy strawberry plants by plate confrontation method,the effect of antagonistic strains on the mycelial growth of the pathogen was observed by light microscope,and the antagonistic bacteria and gray mold pathogen were returned to healthy strawberry fruits at the same time for in vivo inhibition,and their antibacterial effects were evaluated.The analyses of morphological,physiological and biochemical characteristics,and 16S rDNA sequence comparison were performed to identify each antagonistic strain.The results showed that 34 bacterial isolates were isolated and purified from healthy strawberry plants.Among them,five strains with good antagonism performance to strawberry gray mold pathogen were screened through plate confrontation experiments,namely R09,R11,R14,R16 and R22.Their inhibition rates were 67.56%,70.67%,68.11%,65.22%and 63.33%,respectively.Through morphological observation,it was found that all five strains were bacilli with round and opaque colonies,with sizes ranging from 0.321-0.413 μm,0.379-0.582 μm,0.300-0.700 μm,0.500-1.000 μm,and 0.350-0.390 μm,respectively.Based on the morphological,physiological,biochemical,and molecular biological characteristics of each strain,the five endophytic bacteria were identified as Bacillus methylotrophicus,Bacillus siamensis,Bacillus vallismortis,Bacillus velezensis,Bacillus amyloliquefaciens.It was observed that the mycelial morphology of the pathogen in the confrontation area showed obvious deformities,bends,and swelling.The in vivo inhibition test showed that all five antagonistic bacteria had good inhibitory effects on the pathogen of strawberry gray mold,among which Bacillus siamensis had the best effect,the diameters of strawberry lesions was 66.17%smaller than those of CK(without antagoninsti bacteria).This study expanded the strain resources for the biological control of strawberry gray mold and provided a reference for the production of biocontrol agents for strawberry diseases.关键词
草莓/灰霉病/拮抗菌/内生菌/抑菌作用/平板对峙法/系统发育树Key words
Strawberry/Gray mold/Antagonistic bacteria/Endophytes/Bacteriostasis/Plate confrontation method/Phylogenetic tree分类
农业科技引用本文复制引用
马丽,张莉,李成伟,裴冬丽,王芳,郭学良,姚虹宇,刘昊彬,邢小龙,祝春华,齐红志,李献伟,余冬冬..草莓灰霉病菌拮抗内生细菌的分离鉴定及其抑菌作用[J].河南农业科学,2025,54(6):100-109,10.基金项目
河南省教育厅高等学校重点科研项目(24B210010,23B230006,24B230005) （24B210010,23B230006,24B230005）
河南省科技厅科技攻关项目(242102110037) （242102110037）
