农业环境科学学报2025，Vol.44Issue(5)：1160-1168,9.DOI:10.11654/jaes.2024-0744
硫化氢信号分子介导S-烯丙基-L-半胱氨酸调控水稻镉胁迫
Hydrogen sulfide signaling molecules mediate S-allyl-L-cysteine to regulate cadmium stress in rice
摘要
Abstract
Cadmium(Cd)pollution in paddy soil not only affects the normal growth and development of rice,but also brings potential harm to human health when Cd accumulates in grain.As an important signal molecule,hydrogen sulfide(H2S)plays an important role in the regulation of heavy metal stress in plants.In this study,a hydroponic experiment was used to investigate the potential mechanism of S-allyl-L-cysteine(SAC),a garlic extract with H2S donor function,in regulating Cd stress in rice.The main rice variety"ZhongZao 35"planted in southern China was selected as the experimental material and sprayed with SAC at series concentration.The results showed that the H2S content in shoots and roots of rice seedlings continued to increase with time after spraying 0.2 mmol·L-1 SAC,which significantly increased by 44.57％and 83.22％after 12 h compared with the control(CK)treatment.The results of fluorescence labeling on the leaves and roots of rice seedlings also showed that the fluorescence intensity of H2S significantly increased by 254.55％and 700.25％compared with CK treatment.The results of L-cysteine desulfurase(LCD)activity showed that the LCD activities in shoot and root of 0.2 mmol·L-1 SAC were significantly increased by 94.17％and 41.05％compared with CK treatment,respectively.After spraying 0.2 mmol·L-1 SAC,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)in the shoot of rice seedlings were significantly increased by 32.36％,26.69％,24.87％,respectively,the activity in the root was significantly increased by 38.61％,11.99％and 62.93％,respectively,compared with CK treatment.The content of malondialdehyde(MDA)in shoot and root was significantly decreased by 37.37％and 28.54％,respectively.The results showed that when the SAC spraying concentration reached 0.2 mmol·L-1,the Cd contents in shoot and root of rice seedlings were significantly reduced by 18.07％and 46.05％,respectively,compared with CK treatment.In conclusion,SAC spraying triggers the synthesis of H2S signaling molecules and regulates the alleviation of Cd stress in rice.关键词
水稻/镉/S-烯丙基-L-半胱氨酸/硫化氢/信号分子Key words
rice/cadmium/S-allyl-L-cysteine/hydrogen sulfide/signaling molecule分类
农业科技引用本文复制引用
火兴宇,张宇,王常荣,齐麟,刘斌,黄雁飞,黄永春..硫化氢信号分子介导S-烯丙基-L-半胱氨酸调控水稻镉胁迫[J].农业环境科学学报,2025,44(5):1160-1168,9.基金项目
广西创新驱动发展专项资金项目(桂AA22036001-3) （桂AA22036001-3）
国家自然科学基金项目(42077153)Special Fund Project for Guangxi Innovation-Driven Development(AA22036001-3) （42077153）
National Natural Science Foundation of China(42077153) （42077153）
