农业环境科学学报2025，Vol.44Issue(5)：1276-1285,10.DOI:10.11654/jaes.2024-0522
锰离子胁迫下解淀粉芽孢杆菌A-3代谢响应
Metabolic response of Bacillus amyloliquefaciens A-3 under manganese ion stress
摘要
Abstract
In order to study the metabolic response of Bacillus amyloliquefaciens A-3 to Mn2+stress,a sterile fermentation broth of A-3 was used as the research object.All metabolites of A-3 were identified by non-targeted metabolomics technology by adding Mn2+.According to the importance of variable weights(VIP＞1),the differentially expressed metabolites were screened by T-test(P＜0.05),and the A-3 metabolic pathway was enriched by KEGG database.The results showed that Mn2+stress had a significant effect on the changes of metabolites in A-3 fermentation broth.A total of 332 differential metabolites were identified,175 were detected in positive ion mode and 157 were detected in the negative ion mode.Among them,114 metabolites were up-regulated,such as 3-methylindolepyruvate,spermine,adenosine and so on.Down regulation of 218,such as 5-hydroxylysine,citric acid,homogeneous acid and other small analytical organic acids.Through KEGG enrichment and topological analysis of metabolic pathways,22 metabolic pathways with P＜0.05 were found,among which the metabolic pathways of alanine,aspartate and glutamate were most significantly affected.The addition of Mn2+changes the metabolic level of A-3 cells,among which the expression of spermine is up-regulated.Spermine was involved in glutathione metabolism,pantothenate and CoA biosynthesis and other important metabolic and biosynthetic pathways.A-3 mainly responded to Mn2+stress by regulating the expression of amino acids,nucleosides and small molecular organic acids.关键词
Mn2+/解淀粉芽孢杆菌/非靶向代谢组学/代谢物Key words
manganese ion/Bacillus amyloliticus/untargeted metabolomics/metabolite分类
农业科技引用本文复制引用
敖静,李杨,刘晓辉,宋立群,胡琴琴..锰离子胁迫下解淀粉芽孢杆菌A-3代谢响应[J].农业环境科学学报,2025,44(5):1276-1285,10.基金项目
辽宁省国际合作项目(2023JH2/10700013) （2023JH2/10700013）
辽宁省自然科学基金项目(2023-MS-050) （2023-MS-050）
辽宁省重点研发项目(2022020386-JH2/1013)International Cooperation Project of Liaoning Province(2023JH2/10700013) （2022020386-JH2/1013）
National Natural Science Foundation of Liaoning Province(2023-MS-050) （2023-MS-050）
Key Research and Development Project of Liaoning Province(2022020386-JH2/1013) （2022020386-JH2/1013）
