昆明医科大学学报2025，Vol.46Issue(5)：12-20,9.DOI:10.12259/j.issn.2095-610X.S20250502
miR-21-5p靶向STAT3调节OGD/R诱导的神经元损伤
miR-21-5p Targetes STAT3 Reduce the OGD/R-induced Neuronal Injury
摘要
Abstract
Objective To investigate the potential mechanism of miR-21-5p in alleviating cerebral ischemia-reperfusion injury by targeting STAT3.Methods The HT22 cells were induced by OGD/R to construct a cell model of cerebral ischemia reperfusion injury.The expression of miR-21-5p was detected by RT-qPCR.The CCK-8 assay,TUNEL staining and flow cytometry were respectively used to detect the cell viability and apoptosis.ELISA assay was used to determine the contents of inflammatory factors IL-6,IL-10 and TNF-α in the cell supernatant.Western blot was used to detect the expression levels of p-STAT3/STAT3,Cleaved-Caspase-3,Bax and Bcl-2 proteins.The TargetScan database was used to predict the binding sites of miR-21-5p and STAT3.The dual-luciferase reporter gene assay was used to verify the targeting relationship between miR-21-5p and STAT3.Results The relative expression level of miR-21-5p was down-regulated in HT22 cells which induced by OGD/R(P<0.001).The cell viability(P<0.0001)was decreased and the apoptosis rate(P<0.001)was increased in OGD/R induced-HT22 cells.The contents of pro-inflammatory factors IL-6(P<0.001)and TNF-α(P<0.001)was increased,while the content of anti-inflammatory factor IL-10(P<0.001)decreased.After transfection with miR-21-5p mimic,cell viability was enhanced,apoptosis rate was reduced and neuroinflammation was inhibited.MiR-21-5p could target and bind to STAT3.After miR-21-5p inhibitor transfection,cell viability decreased,apoptosis was promoted,and neuroinflammation occurred;STAT3 inhibitor Stattic could reverse the effect of miR-21-5p inhibitor.Conclusion MiR-21-5p could specifically bind to STAT3 and reduce the neuroinflammation and apoptosis of OGD/R induced-HT22 cells.关键词
脑缺血再灌注损伤/miR-21-5p/STAT3/OGD/R/凋亡/神经炎症Key words
Cerebral ischemia reperfusion injury/miR-21-5p/STAT3/Oxygen-glucose deprivation/reoxygenation/Apoptosis/Neuroinflammation分类
医药卫生引用本文复制引用
李妍平,李青芸,霍蓉,董小林,李红梅,魏欢,曾毅..miR-21-5p靶向STAT3调节OGD/R诱导的神经元损伤[J].昆明医科大学学报,2025,46(5):12-20,9.基金项目
云南省科技厅-昆明医科大学基础研究联合专项基金(202401AY070001-184) （202401AY070001-184）
云南省教育厅科学研究基金(2023Y0670/2023J0283) （2023Y0670/2023J0283）
昆明市卫生健康委员会卫生科研课题(2023-03-07-006) （2023-03-07-006）
昆明市卫生科技人才培养项目医学科技学科后备人才培养计划[2023-SW(后备)-47] （后备）
