昆明医科大学学报2025，Vol.46Issue(5)：30-37,8.DOI:10.12259/j.issn.2095-610X.S20250504
槲皮素对BMSCs成骨分化的影响及作用机制
Effects and Mechanism of Quercetin on Osteogenic Differentiation of BMSCs
摘要
Abstract
Objective To explore the effect and mechanism of quercetin on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods BMSCs were divided into a blank control group(Control)and quercetin(Quercetin)low-dose group(4.8 mL/kg),medium-dose group(9.6 mL/kg),and high-dose group(19.2 mL/kg)intervened with drug-containing serum,while the positive control group was treated with osteogenic differentiation medium,respectively.The cell cycle was analysed by flow cytometry,cell proliferation was detected by MTT assay,cell activity was determined by Alkaline phosphatase(ALP)assay kit,and calcified nodule formation was observed by alizarin red staining.The expression of β-catenin and the key factors of osteogenic differentiation,runt-related transcription factor 2(RUNX2)and osteocalcin(OCN),were detected by qPCR and Western blot,respectively.Results Compared with the control group,quercetin-containing serum significantly promoted the proliferation of BMSCs(P=0.000205,P=0.000063)and enhanced the formation of calcium nodules,and increased osteogenic and ALP activity after osteogenic differentiation.The results of qPCR and Western blot showed that the quercetin group significantly up-regulated the mRNA and protein expression of β-catenin(P<0.0001),RUNX2(P<0.0001)and OCN(P<0.0001)during osteogenic differentiation.Conclusion Quercetin can effectively promote the osteogenic differentiation of BMSCs,and its mechanism is achieved by activating the Wnt/β-catenin signaling pathway and up-regulating the expression of the osteogenesis-related transcription factor RUNX2.关键词
槲皮素/骨髓间充质干细胞/成骨分化/Wnt/β-cateninKey words
Quercetin/Bone marrow mesenchymal stem cells/Osteogenic differentiation/Wnt/β-catenin分类
临床医学引用本文复制引用
徐静逍,刘佳,姚姝,张希,李江,崔桂琴,易小玲,李东云..槲皮素对BMSCs成骨分化的影响及作用机制[J].昆明医科大学学报,2025,46(5):30-37,8.基金项目
云南省科技厅中医联合专项-青年项目(202101AZ070001-132) （202101AZ070001-132）
(202101AZ070001-130) （202101AZ070001-130）
(202101AZ070001-319) （202101AZ070001-319）
昆明市卫生健康委员会卫生科研课题项目(2022-16-01-008) （2022-16-01-008）
