生物灾害科学2025，Vol.48Issue(2)：235-244,10.DOI:10.3969/j.issn.2095-3704.2025.02.30
基于16S Rdna高通量测序探讨弓形虫对鸡肠道微生物的影响
The impact of Toxoplasma gondii on chicken gut microbiota based on 16S rDNA high-throughput sequencing
摘要
Abstract
[Objective]This study aimed to investigate the impact of Toxoplasma gondii infection on the gut microbiota of chickens,providing a theoretical basis for elucidating the interaction between Toxoplasma and intestinal flora,and offering new insights for the prevention and treatment of toxoplasmosis.[Method]Twenty 7-day-old and ten 28-day-old healthy chickens were divided into infected and control groups.The cecal microbial composition was analyzed by using 16S rDNA high-throughput sequencing on day 11 post-infection.[Result]Differences in microbial diversity were observed between infected and control groups.Compared to the 7-day-old control group,the 7-day-old infected group exhibited a significant increase in the abundance of Firmicutes(P<0.05)and a significant decrease in Actinobacteria(P<0.05).At the genus level,Lactobacillus and Bifidobacterium were highly significantly reduced(P<0.01),while Bacteroides increased significantly(P<0.05).In the 28-day-old infected group,the abundance of Bacteroidetes significantly increased(P<0.05).At the genus level,Lactobacillus was highly significantly reduced(P<0.01),whereas Bacteroides showed a highly significant elevation(P<0.01).Additionally,the relative abundance of Lactobacillus in the 7-day-old infected group was higher than that in the 28-day-old infected group,while Bacteroides exhibited the opposite trend.[Conclusion]These findings suggest that Toxoplasma gondii infection alters the gut microbial composition in chickens,with more severe effects on chicks,potentially leading to higher mortality rates.关键词
弓形虫/肉鸡/16S rDNA/肠道微生物Key words
Toxoplasma gondii/broiler chicken/16S rDNA/gut microbe分类
畜牧业引用本文复制引用
邓晓瑛,石佳佳,杨成昊,鲁超,陈小庆..基于16S Rdna高通量测序探讨弓形虫对鸡肠道微生物的影响[J].生物灾害科学,2025,48(2):235-244,10.基金项目
国家自然科学基金项目(32002288)和2024 年国家级大学生创新创业训练计划项目(202410410006X) （32002288）
