四川农业大学学报2025，Vol.43Issue(3)：551-563,13.DOI:10.16036/j.issn.1000-2650.202412523
小麦农家种笨蚰子麦成株期条锈病抗性基因分子鉴定及其潜在育种应用价值评价
Molecular Identification and Evaluation of Potential Breeding Application of Adult Plant Stripe Rust Resistance Genes in Chinese Wheat Landrace Benyouzi
摘要
Abstract
[Objective]Previous studies have demonstrated that the Chinese wheat landrace Benyouzi(BYZ)exhibits precocity,effective multi-tillering,superior grain filling,and stable adult-plant resis-tance to dominant physiological races and pathological types of wheat stripe rust(Puccinia striiformis f.sp.tritici)in China.These traits highlight its potential as an elite germplasm for enhancing wheat yield and stripe rust resistance.However,the genetic basis of adult-stage resistance and its synergistic effects with yield traits remain unclear.This study aims to identify stripe rust resistance genes in Benyouzi and elucidate their role in coordinating disease resistance and yield improvement.[Method]A genetic map-ping population was developed by crossing the resistant parent Benyouzi,which exhibits strong adult-stage resistance to stripe rust,with the susceptible parent Avocet S.Phenotypic evaluations and genetic analyses of stripe rust resistance were conducted using mixed races of Pst.Bulked segregant analysis com-bined with exome capture sequencing(BSE-seq)was employed to identify adult-plant stripe rust resis-tance genes in BYZ,and molecular markers for marker-assisted selection(MAS)were developed.Addi-tionally,a BC1F2 genetic improvement population derived from Xikemai 5(XKM5)×BYZ was con-structed to investigate the genetic effects of BYZ-derived resistance genes on disease resistance and yield traits.[Result]Genetic analysis of resistance phenotypes indicated that adult-plant stripe rust resis-tance in BYZ is controlled by multiple genes.Two major quantitative trait loci(QTLs),designated QYr.BYZ-5BS and QYr.BYZ-7DS,were identified on chromosomes 5BS and 7DS using BSE-seq.These QTLs accounted for 9.06％-11.07％and 27.36％-29.90％of phenotypic variation,respectively.Further analysis confirmed that the resistance effect of QYr.BYZ-7DS is conferred by the known adult-plant resis-tance gene Yr18,while QYr.BYZ-5BS represents a potentially novel resistance locus.A competitive allele-specific PCR(KASP)marker,KP5B_83.68,was developed for QYr.BYZ-5BS.Evaluation of the BC1F2 population revealed that the presence or pyramiding of QYr.BYZ-5BS and Yr18 significantly in-creased tiller number,reduced plant height,and effectively mitigated stripe rust infection.[Conclu-sion]A novel QTL(QYr.BYZ-5BS)controlling adult-plant stripe rust resistance was identified in the Chinese wheat landrace BYZ,and a KASP marker(KP5B_83.68)for its molecular tracking was devel-oped.Genetic analyses suggest that QYr.BYZ-5BS holds significant potential for synergistically improv-ing disease resistance and yield traits in wheat breeding programs.关键词
小麦农家品种/成株期条锈病抗性基因/回交改良/产量相关性状/遗传效应Key words
wheat landrace/adult plant stripe rust resistance genes/backcrossed improvement/yield-related traits/genetic effects分类
农业科技引用本文复制引用
邓梅,管方念,龙黎,张潇月,高梦茹,李豪,魏育明,蒋云峰,陈国跃..小麦农家种笨蚰子麦成株期条锈病抗性基因分子鉴定及其潜在育种应用价值评价[J].四川农业大学学报,2025,43(3):551-563,13.基金项目
国家重点研发计划(2024YFD1201200) （2024YFD1201200）
国家农业重大科技专项(NK20220607) （NK20220607）
国家自然科学基金(32272059) （32272059）
四川省重大科技专项(2022ZDZX0014) （2022ZDZX0014）
四川省自然科学基金(2024NSFSC0318、2024NSFSC1842、2024NSFSC1212). （2024NSFSC0318、2024NSFSC1842、2024NSFSC1212）
