四川农业大学学报2025，Vol.43Issue(3)：564-571,8.DOI:10.16036/j.issn.1000-2650.202411535
水稻异倍性杂交种子发育和胚拯救研究
Research on Seed Development and Embryo Rescue in Aneuploid Hybrids Rice
摘要
Abstract
[Objective]This study investigates endosperm and embryo development in interploidy hybrid rice seeds to elucidate the embryological mechanisms underlying seed abortion induced by parental ge-nome dosage imbalance.[Method]Reciprocal crosses were conducted using diploid(2n)indica rice(Haitian),diploid(2n)japonica rice(Nipponbare),and tetraploid(4n)Haitian.Endosperm develop-ment in hybrid progeny was observed,and immature embryos were rescued to obtain offspring.Ploidy lev-els of rescued plants were determined via chromosome squashing,and SSR molecular markers verified hybrid authenticity.[Result]① Heteroploid hybrid seeds exhibited variations in seed size and endo-sperm development.② Embryo rescue enabled recovery of heteroploid hybrid seeds,though triploid em-bryos displayed weaker growth compared to diploid and tetraploid counterparts.③ Germination rates of triploid embryo-rescued progeny differed between reciprocal crosses,and rescued plants were sterile.[Conclusion]Seed abortion in interploidy rice hybrids arises from asynchronous endosperm cellulariza-tion,impairing nutrient transfer to embryos and inducing abortion.While in vitro embryo rescue yields seedlings,their growth vigor remains significantly compromised.关键词
水稻/倍性/胚乳发育/幼胚拯救Key words
rice/ploidy/endosperm development/young embryo rescue分类
农业科技引用本文复制引用
郑海燕,吴先军,张红宇,朱晓炜,王延新,杨甦婷,王振奇,刘佳缘,胡杰,高清熠,刘磊..水稻异倍性杂交种子发育和胚拯救研究[J].四川农业大学学报,2025,43(3):564-571,8.基金项目
国家科技重大专项-高产新基因挖掘与育种价值评价(2023ZD04068). （2023ZD04068）
