四川农业大学学报2025，Vol.43Issue(3)：588-596,617,10.DOI:10.16036/j.issn.1000-2650.202501529
茉莉酸甲酯调控花生类黄酮生物合成相关基因的研究
Study on Methyl Jasmonate-Mediated Regulation of Flavonoid Biosynthesis-Related Genes in Peanut(Arachis hypogaea)
摘要
Abstract
[Objective]To investigate the antioxidant effects of flavonoids in peanut cultivar Kainong 1760 under environmental stress and its stress resistance mechanisms.[Method]Peanut seedlings were treated with methyl jasmonate(MeJA)for 0,2,4,and 12 h to simulate stress conditions.Total flavonoid content was measured,followed by transcriptome sequencing and drought stress experiments.MeJA was applied to mitigate drought effects.[Result]The results showed that 796 genes were significantly co-differentially induced by MeJA.The total flavonoid content in peanuts significantly increased and peaked at 2 h of MeJA treatment(JA2).Differential expression gene(DEG)analysis revealed that the KEGG enrichment in the JA2 samples primarily regulated the phenylpropanoid and the isoflavone meta-bolic pathways,involving catalytic enzymes related to lignin and its derivatives.For drought-stressed seedlings,the application of MeJA promoted the increase in superoxide dismutase(SOD),peroxidase(POD),the osmolyte proline(Pro),and malondialdehyde(MDA)content,while reducing hydrogen perox-ide(H2O2)content.Fluorescence quantitative analysis showed that the relative expression levels of differ-entially expressed genes were consistent with the gene expression trends in the transcriptome sequencing results.[Conclusion]This study provides a theoretical foundation and basis for understanding the influ-ence of MeJA signaling on flavonoid metabolism and stress resistance breeding in peanuts.关键词
花生/黄酮类/茉莉酸甲酯/代谢通路/基因调控网络Key words
peanut/flavonoids/methyl jasmonate/metabolic pathway/gene regulatory network分类
农业科技引用本文复制引用
苗建利,邓丽,王培云,郭敏杰,李阳,殷君华,胡俊平,任丽..茉莉酸甲酯调控花生类黄酮生物合成相关基因的研究[J].四川农业大学学报,2025,43(3):588-596,617,10.基金项目
国家现代农业产业技术体系(CARS-13) （CARS-13）
河南省农业良种攻关项目(20220100001) （20220100001）
开封市重点研发专项(22ZDYF008). （22ZDYF008）
