四川农业大学学报2025，Vol.43Issue(3)：597-606,10.DOI:10.16036/j.issn.1000-2650.202502503
油菜BnMPK5激酶基因的克隆及其在渍害胁迫下的功能分析
The Cloning and Functional Analysis of the BnMPK5 Kinase Gene upon Waterlogging Stress in Brassica napus
摘要
Abstract
[Objective]This study aimed to identify key genes involved in regulating waterlogging stress responses in rapeseed(Brassica napus)and to elucidate the biological function of BnMPK5 in waterlog-ging tolerance.[Method]Transcriptome analysis was performed to identify differentially expressed genes(DEG)in Arabidopsis thaliana under waterlogging conditions.And the homologous genes of these DEGs in rapeseed were then examined for their response to waterlogging stress.The key regulatory gene BnMPK5 was cloned,and its functional role in waterlogging tolerance was further investigated.[Result]Two AtMPK5 homologs,BnA09MPK5 and BnC09MPK5,were identified in rapeseed.Among them,the ex-pression of BnC09MPK5 quickly reduced under waterlogging stress.After 4 hours relief,the expression of BnC09MPK5 recovered to normal level.Moreover,The BnMPK5 overexpression lines were created in Arabidopsis to investigate its biological function upon waterlogging stress.Consequently,under waterlog-ging treatment,the BnMPK5 overexpression lines exhibited clearer senescence phenotype,including yel-low and pale leaves.Additionally,the level of H2O2,O-2,and MDA in the BnMPK5 overexpression lines were increased by 70%,92%,and 45%,respectively,while electrolyte leakage rose by 39%,when compared to the untreated plants.[Conclusion]In rapeseed,the expression of BnMPK5 was tightly con-trolled by waterlogging stress.Our study validated that BnMPK5 play a negative role in response to water-logging stress,via disrupting reactive oxygen species(ROS)homeostasis and exacerbating membrane lipid peroxidation damage.It highlights BnMPK5 serves as a potential molecular target for unraveling the mechanisms of waterlogging tolerance and advancing stress-resistant breeding in rapeseed.关键词
渍害/油菜/克隆/BnMPK5/转录组分析Key words
waterlogging stress/Brassica napus/clone/BnMPK5/transcriptome analysis分类
农业科技引用本文复制引用
余霆杰,郑倩倩,龚容,彭浈,林子冰,张毅,柴靓,李婷..油菜BnMPK5激酶基因的克隆及其在渍害胁迫下的功能分析[J].四川农业大学学报,2025,43(3):597-606,10.基金项目
粮油作物绿色种质创新与遗传改良四川省重点实验室开放课题(2023LYKF04) （2023LYKF04）
国家现代农业产业技术体系四川油菜创新团队(SCCXTD-2024-3). （SCCXTD-2024-3）
