四川农业大学学报2025，Vol.43Issue(3)：630-640,668,12.DOI:10.16036/j.issn.1000-2650.202504571
不同水分条件下夏栎生长生理和转录组分析
Physiology and Transcriptome Analysis of Quercus robur under Different Moisture Conditions
摘要
Abstract
[Objective]To investigate the effects of different water conditions on growth-physiological characteristics and gene expression in Quercus robur seedlings,thereby providing scientific guidance for cultivation management,genetic improvement and ecological conservation of Q.robur.[Method]Two-year-old Q.robur seedlings were subjected to five soil water gradients using pot-controlled water treat-ments,containing T1:20%field holding capacity(FCW);T2:40%FCW;T3:60%FCW;T4:80%FCW;T5:100%FCW.Growth and physiological parameters of Q.robur were measured and leaf tran-scriptome sequencing was carried out after 90 d of treatment.[Result]All seedlings survived and con-tinued growing across water regimes,showing 26.39%～59.46%and 9.16%～59.06%,reductions in height and basal diameter growth respectively with decreasing soil moisture;The activities of leaf super-oxide dismutase(SOD)and catalase(CAT)increased by 18.70%～56.98%and 65.04%～121.14%,while the activities of peroxidase(POD)and catalase(POD)increased by 18.70%～56.98%and 65.04%～121.14%.Whereas,the activities of peroxidase(POD)and ascorbate peroxidase(APX)were decreased by 20.41%～75.51%,7.84%～35.29%;the contents of malondialdehyde(MDA),hydrogen peroxide(H2O2)and proline(Pro)in the leaves of Q.robur were increased under T1 treatment by 20.68%～65.51%,137.50%～800.00%,and 16.50%to 107.25%,whereas the content of soluble sugars(SS)increased by 15.46%to 54.76%and soluble proteins(SP)decreased by 6.06%to 42.42%with moisture increment.Transcriptome analysis identified 605 differentially expressed genes(DEGs)under different moisture gradients.These DEGs were predominantly enriched in sesquiterpenes,triterpenes and phenylpropanes biosynthesis pathways.In addition,key genes related to phenylpropanoid pathway,including phenylalanine deaminase(PAL),cinnamoyl coenzyme A reductase(CCR),and cinnamyl al-cohol dehydrogenase(CAD),showed significant expression modulation.[Conclusion]Reduced soil moisture negatively impacts the growth of Q.robur.Q.robur leaves maintain intracellular osmotic homeo-stasis by accumulating osmoregulatory substances(Pro),activating antioxidant enzyme systems(CAT,SOD)for effective scavenging of reactive oxygen species and reducing membrane lipid peroxidation,thus enhancing its tolerance.In addition,the expression of PAL gene in the phenylpropane biosynthesis path-way was significantly induced by the reduction of soil moisture,suggesting that this pathway may play an important role in the physiological adaptation of Q.robur in response to the changes of different moisture gradients,which provides a theoretical basis for the in-depth understanding of Q.robur's water adapta-tion mechanism and the screening of key functional genes.关键词
夏栎/不同水分处理/生长生理特性/转录组分析Key words
Quercus robur/different water treatments/eophysiological characteristics/transcriptome analysis分类
农业科技引用本文复制引用
刘嘉,豆梦珂,李元会,胡秋红,陈睿萱,甘胜美,范江涛,黄雄..不同水分条件下夏栎生长生理和转录组分析[J].四川农业大学学报,2025,43(3):630-640,668,12.基金项目
2023年大学生创新创业训练计划项目(S202310626025) （S202310626025）
四川省重点研发计划项目(2024YFNH0028). （2024YFNH0028）
