上海针灸杂志2025，Vol.44Issue(6)：750-759,10.DOI:10.13460/j.issn.1005-0957.2024.13.3038
针灸对溃疡性结肠炎大鼠DNA羟甲基化酶TETs介导免疫调节的影响
Effect of acupuncture and moxibustion on immunoregulation mediated by DNA hydroxymethylases TETs in rats with ulcerative colitis
摘要
Abstract
Objective Recurrent chronic inflammation in ulcerative colitis(UC)may cause cancer.This study observed the protective effect of mild moxibustion and electroacupuncture(EA)on the intestinal mucosa of UC rats and the immunoregulatory mechanism.Method Sprague-Dawley(SD)rats were randomized into a normal group,a model group,a mild moxibustion group,an EA group,and a Western medicine group.The UC model was established by intraperitoneal injection of Azoxymethane(AOM)(10 mg/kg)plus freely drinking 5%Dextran sulfate sodium salt(DSS)solution for the subsequent 5 consecutive days.The general condition,colon length,and colonic histopathology and score were observed.The enzyme-linked immunosorbent assay(ELISA)was adopted to detect the serum contents of interleukin-6(IL-6)and transforming growth factor beta 1(TGF-β1);the immunofluorescence(IF)technique was used to locate 5-methylcytosine(5-mC),5-hydroxymethylcytosine(5-hmC),ten-eleven translocation(TET)family proteins,and histone deacetylase 2(HDAC2)and detect their expression in the colonic tissue.Result Compared to the normal group,the model group had a significantly shorter colon length and an increased colonic histopathological score(P＜0.05),along with an elevated IL-6 content and a decreased TGF-β1 content in the serum(P＜0.05),increased 5-mC protein expression(P＜0.05),and reduced protein expression of 5-hmC,TET1,TET2,TET3,and HDAC2(P＜0.05).Compared to the model group,the mild moxibustion,EA,and Western medicine groups all demonstrated an increased colon length and a declined pathological score(P＜0.05),together with an elevated TGF-β1 content and a decreased IL-6 content in the serum(P＜0.05),increased protein expression of 5-hmC,TET1,TET2,TET3,and HDAC2 in the colonic tissue(P＜0.05);the mild moxibustion group also showed decreased colonic 5-mC protein expression(P＜0.05).In the mild moxibustion group,TET1,TET2,and TET3 were found co-expressed with HDAC2 in the colon;TET2 and TET3 co-expressed with HDAC2 in the EA group;TET1 and TET3 were found co-expressed with HDAC2 in the Western medicine group.Conclusion Both mild moxibustion and EA can produce protective effects on colonic mucosa in UC,and the mechanism should be related to their effects on up-regulating the protein expression of DNA hydroxymethylases TETs and HDAC2 and maintaining the 5-mC/5-hmC balance.Further,mild moxibustion can recruit HDAC2 via TET1,TET2,and TET3,while EA only via TET2 and TET3,to play the immunoregulatory role.关键词
结肠炎,溃疡/温和灸/电针/DNA羟甲基化酶TETs/白介素6/5-甲基胞嘧啶/大鼠Key words
Colitis,Ulcerative/Mild moxibustion/Electroacupuncture/DNA hydroxymethylases TETs/Interleukin-6/5-methylcytosine/Rats分类
医药卫生引用本文复制引用
张玥,李峰,吴焕淦,黄艳,沈亚,朱璐,徐静,杨玲,赵继梦,马喆,祁琴,冯格格..针灸对溃疡性结肠炎大鼠DNA羟甲基化酶TETs介导免疫调节的影响[J].上海针灸杂志,2025,44(6):750-759,10.基金项目
国家自然科学基金(82105012,81873372,82074546) （82105012,81873372,82074546）
上海市自然科学基金(22ZR1458400) （22ZR1458400）
上海市卫生健康委员会卫生行业临床研究专项(202140113) （202140113）
上海市人才发展基金资助项目(2021058) （2021058）
