食管疾病2025，Vol.7Issue(2)：81-87,7.DOI:10.15926/j.cnki.issn2096-7381.2025.02.001
利用苛养厌氧平板分离牙龈卟啉单胞菌临床菌株及其生物学特性分析
Isolation of Clinical Strains of Porphyromonas gingivalis Using Fastidious Agaerobic Agar and Analysis of Their Biological Characteristics
摘要
Abstract
Objective To select a solid agar medium suitable for the growth of Porphyromonas gingivalis(Pg)and to establish an effective and rapid method for the isolation of Pg strains.Methods By observing the growth cycle and colony characteristics of Pg,we investigated the growth characteristics of Pg standard strain ATCC 33277 on fastidious anaerobic agar(FAA),tryptic soy peptone agar(TSA),and bovine brain heart infusion agar(BHIA).BHIA were used to characterize the growth and screen the best plates.Dental plaque was collected from 1 patient with chronic periodontitis in an area with high incidence of esophageal cancer,and fresh tumor tissue from 3 patients with esophageal squamous cell carcinoma was collected,identi-fied as positive for Pg by real-time fluorescence quantitative PCR(qPCR),and cultured on FAA plates with continuous lines for routine anaerobic culture.Gram staining,qPCR and 16S rRNA sequencing were used to preliminarily identify the isolated clinical strains,and experiments such as growth curve test,drug sensitivity and biofilm-forming ability were carried out to further study the biological characteristics of the isolated clinical strains.Results Compared with BHIA and TSA,FAA was more suitable for the growth of Pg and its sensitivity was higher.A clinical strain of Pg,named LyG1,was successfully isolated from oral cavity,and a clinical strain of Pg,named LyEC01,was isolated from esophageal squamous carcinoma tissues.Conclusion FAA is suitable for the isolation,purification as well as cultivation of Pg,and the clinical strains of LyG1 and LyEC01,which were isolated from oral cavity and esophageal carcinoma tissues,differed from the standard strains in their biological characteristics.关键词
食管癌/牙龈卟啉单胞菌/苛养厌氧平板/临床菌株Key words
esophageal cancer/Porphyromonas gingivalis/fastidious anaerobic agar/clinical strains分类
临床医学引用本文复制引用
杨睿,裴广坤,石林林,左志向,颜贞,谷变利,高社干..利用苛养厌氧平板分离牙龈卟啉单胞菌临床菌株及其生物学特性分析[J].食管疾病,2025,7(2):81-87,7.基金项目
河南省重点研发与推广专项(科技攻关)项目(222102310115)河南省医学科技攻关省部共建重点项目(SBGJ202102199) （科技攻关）
