水生生物学报2025，Vol.49Issue(7)：112-121,10.DOI:10.7541/2025.2024.0389
乙醇梭菌蛋白替代鱼粉对日本沼虾生长及代谢的影响
CLOSTRIDIUM AUTOETHANOGENUM PROTEIN REPLACING FISHMEAL ON GROWTH PERFORMANCE AND METABOLISM OF MARCOBRACHIUM NIPPONENSE
摘要
Abstract
The discovery and developing of new protein feedstuff have always been an important research focus in aquatic feed industry.To evaluate the effects of new protein feedstuff Clostridium autoethanogenum protein(CAP)in aquatic feed,oriental river prawn(Macrobrachium nipponense)was studied to detect the growth performance,protein and lipid metabolism when it was fed diet with CAP replacing fishmeal(FM).Six isonitrogen and isolipids diets were formulated including D1(control group with 25%FM),D2(CAP replacing 10%FM),D3(CAP replacing 20%FM),D4(CAP replacing 30%FM),D5(CAP replacing 40%FM),and D6(CAP replacing 50%FM).A total of 1200 orien-tal river prawns[average initial body weight(0.102±0.001)g]were randomly divided into six treatments with four replicates.The feeding trail lasted for 8 weeks.Results showed no significant differences in survival rate of prawns in each treatment(P>0.05).The weight gain rate(WGR),specific growth rate(WGR),feed coefficient ratio(FCR),and protein efficiency ratio(PER)of prawns fed diet with CAP replacing 20%FM were not significantly different from those in the control group(P>0.05).However,when CAP replaced more than 20%FM,the WGR,SGR,and PER significantly decreased,while FCR significantly increased compared to the control group(P<0.05).The whole body composition of prawns,including moisture,crude protein,lipid,and ash,was not significantly affected by the diet with CAP replacing FM(P>0.05).Notably,the non-essential amino acid content,total amino acid content,and especially glycine content in the prawn muscle significantly increased when CAP partially replaced FM in the diet(P<0.05).The total hemolymph protein of the prawn fed diet with CAP replacing 10%FM was not significantly different with that of the control group(P>0.05).The hepatopancreatic lipase activities were significantly higher in the D2 group than those in the control group(P<0.05).However,hemolymph total cholesterol and triglycerides were significantly lower,while hemolymph glucose was significantly higher in the prawns fed diet with CAP replacing more than 10%FM compared to that in the control group(P<0.05).Gene expression analysis revealed that the relative expression of tor and sr-bⅠsignificantly up-regulated in the prawns fed diet with CAP replacing 20%FM than that in the control group(P<0.05).Moreover,when CAP replaced more than 20%FM,the relative expressions of s6k1,atf4,acc,fas,and hsl were up-regulated significantly compared to the D2 group(P<0.05).Except for the tor and sr-bⅠ genes in D3 group and the fas gene in D6 group,the relative expressions of genes related to protein and lipid metabolism in the hepatopancreas showed no significantly difference from those of the control group(P>0.05).In conclusion,CAP can replace about 20%FM in the diet of M.nipponense without negative effect on growth performance.CAP holds potential as a novel feed ingredient in the aquaculture industry.关键词
乙醇梭菌蛋白/鱼粉替代/生长/营养代谢/肝胰腺/基因表达/日本沼虾Key words
Clostridium autoethanogenum protein/Fishmeal replacement/Growth performance/Nutrient metabolism/Hepatopancreas/Gene expression/Oriental river prawn分类
水产学引用本文复制引用
高国栋,杨昶,Momodou Saidyleigh,刘波,潘良坤,夏冬,孙存鑫,郑肖川,周群兰..乙醇梭菌蛋白替代鱼粉对日本沼虾生长及代谢的影响[J].水生生物学报,2025,49(7):112-121,10.基金项目
江苏省面上项目"5-HT1B/5-HT 轴负反馈调控日本沼虾的打斗行为的机制研究"(BK20231138) （BK20231138）
江苏现代农业(青虾)产业技术体系营养与饲料创新团队(JATS[2023]470) （青虾）
国家虾蟹产业技术体系淡水虾营养与饲料岗位(CARS-48)资助[Supported by the Natural Science Foundation of Jiangsu Province(BK20231138) （CARS-48）
the Earmarked Fund for Jiangsu Agricultural Industry Tech-nology System(JATS[2023]470) （JATS[2023]470）
China Agriculture Research System of MOF and MARA(CARS-48)] （CARS-48）
