水生生物学报2025，Vol.49Issue(7)：156-166,11.DOI:10.7541/2025.2024.0458
鲢粪便对湖泊沉积物N2O通量与微生物群落特征的影响
EFFECTS OF HYPOPHTHALMICHTHYS MOLITRIX FAECES ON N2O FLUXES AND MICROBIAL COMMUNITY CHARACTERISTICS IN LAKE SEDIMENTS
摘要
Abstract
Filter-feeding fish play an important role in coping with the eutrophication process of water bodies.In order to understand the effect of filter-feeding fish faeces on N2O emissions from lake sediments,we collected sediments,water,and feces of Hypophthalmichthys molitrix in Qiandao Lake,and set up three experimental groups according to different ratios.A 90-day simulated closed culture transformation experiment was carried out.The key microbial strains affecting nitrogen transformation and the main environmental factors influencing nitrogen transformation were identi-fied by analysing the emission flux characteristics of N2O,the physicochemical indexes of water and sediment mixtures,nitrogen metabolism enzyme activities,and microbial sequences.The results showed that under the influence of fish faeces,the N2O emission flux at the water-gas interface reached the highest on day 45 and then decreased rapidly.From day 45 to day 60,the flux shifted from emission to uptake.The greater the proportion of fish faeces added to the sediment,the greater the uptake of N2O during the later stages of incubation.The addition of fish faeces significantly increased the concentration of soluble carbon and ammoniacal nitrogen in the recirculation system,while decreasing DO in the water column.It also significantly enhanced the activities of four relevant nitrogen-converting enzymes in the sediments.16sRNA analysis of the sediments showed that the addition of fish manure significantly increased the relative abundance of Proteobacteria,Firmicutes,and Bacteroidota.The increased abundance of Bacillus probably being the main reason for the sediments to show stronger N2O uptake efficiency.This study provides a theo-retical reference for the N2O abatement mechanism of filter-feeding fish proliferation in lakes.关键词
滤食性鱼类/千岛湖沉积物/N2O排放通量/氮循环/微生物机制/鲢Key words
Filter-feeding fish/Lake sediments/N2O emission flux/Nitrogen cycle/Microbiological mechanism/Hypophthalmichthys molitrix分类
农业科技引用本文复制引用
陈宏远,刘慧仪,车轩,盘家永,李一鸣,王婕,朱林,孟展鹏,卢小利,崔帅康..鲢粪便对湖泊沉积物N2O通量与微生物群落特征的影响[J].水生生物学报,2025,49(7):156-166,11.基金项目
国家重点研发计划(2023YFD2400905) （2023YFD2400905）
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金CAFS(2023TD67)资助[Supported by National Key Research And Development Program of China(2023YFD2400905) （2023TD67）
Central Public-interest Scientific Institution Basal Research Fund,CAFS(2023TD67)] （2023TD67）
